In cellulo structure determination of a novel cypovirus polyhedrin. (1st May 2014)

• Record Type: Journal Article
• Authors: Axford, Danny; Ji, Xiaoyun; Stuart, David I.; Sutton, Geoff
• Is Part Of: Acta crystallographica. Volume 70:Part 5(2014:May)
• Page Start: 1435
• Page End: 1441
• Publication Date: 2014-05-01
• Subjects: Biomolecules -- Structure -- Periodicals; Physical biochemistry -- Periodicals; X-ray crystallography -- Periodicals; Crystallography -- Periodicals; 572
• DOI: 10.1107/S1399004714004714
• Languages: English
• ISSNs: 0907-4449

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>This work demonstrates that with the use of a microfocus synchrotron beam the structure of a novel viral polyhedrin could be successfully determined from microcrystals within cells, removing the preparatory step of sample isolation and maintaining a favourable biological environment. The data obtained are of high quality, comparable to that obtained from isolated crystals, and enabled a facile structure determination. A small but significant difference is observed between the unit‐cell parameters and the mosaic spread of <italic>in cellulo</italic> and isolated crystals, suggesting that even these robust crystals are adversely affected by removal from the cell.</p> </abstract>