Inactivation of Patched1 in Murine Chondrocytes Causes Spinal Fusion Without Inflammation. Issue 4 (April 2014)

• Record Type: Journal Article
• Authors: Dittmann, Kai; Wuelling, Manuela; Uhmann, Anja; Dullin, Christian; Hahn, Heidi; Schweyer, Stefan; Vortkamp, Andrea; Wienands, Jürgen
• Is Part Of: Arthritis & rheumatology. Volume 66:Issue 4(2014)
• Page Start: 831
• Page End: 840
• Publication Date: 2014-04
• Subjects: Arthritis -- Periodicals; Rheumatism -- Periodicals; 616.72
• DOI: 10.1002/art.38325
• Languages: English
• ISSNs: 2326-5191

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="art38325-sec-0001" sec-type="section"> <title>Objective</title> <p>During development of the vertebrate skeleton, chondrocytes form a cartilage template that is gradually replaced by bone. Hormones of the Hedgehog (HH) family have been implicated in the ossification process, but their exact relationship to normal or pathogenic bone formation is unclear. This study was undertaken to establish a genetic tool that allows the discrete inactivation of genes in spinal chondrocytes, and to investigate in vivo how chondrocyte‐specific ablation of the inhibitory HH receptor Patched 1 (<italic>Ptch1)</italic> affects skeleton integrity.</p> </sec> <sec id="art38325-sec-0002" sec-type="section"> <title>Methods</title> <p>A <italic>Cre</italic>‐deleter mouse strain, <italic>mb1‐Cre</italic>, for selective gene recombination in spinal chondrocytes was identified by in situ hybridization and histologic analysis. The <italic>mb1‐Cre</italic><sup>+/−</sup> animals were crossed with mice that harbor a loxP‐flanked <italic>Ptch1</italic> gene (<italic>Ptch1</italic><sup><italic>flox/flox</italic></sup>) to abrogate the inhibition of the HH signaling pathway in chondrocytes. The skeletal integrity of F1 mice was characterized by high‐resolution flat‐panel–based volume computed tomography and histologic staining procedures.</p> </sec> <sec id="art38325-sec-0003" sec-type="section"> <title>Results</title> <p>During the first weeks after birth, all <italic>mb1‐Cre</italic><sup>+/−</sup>/<italic>Ptch1</italic><sup><italic>flox/flox</italic></sup> mice developed progressive spinal fusion with malformation of the vertebrae. This phenotype was caused by aberrant chondrocyte proliferation in the intervertebral discs that blocked endochondral ossification. Importantly, the disease pattern occurred in an inflammation‐independent manner.</p> </sec> <sec id="art38325-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Our findings indicate that chronic activation of the HH signal pathway in spinal chondrocytes can trigger an ankylosing spine morphology without immune cell contributions. Hence, the destruction of cartilage and loss of axial joint integrity can result from chondrocyte‐intrinsic defects of monogenic origin.</p> </sec> </abstract>