New biomaterial based on cotton with incorporated Biomolecules. Issue 15 (18th February 2014)

• Record Type: Journal Article
• Authors: Gomes, Ana P.; Mano, João F.; Queiroz, João A.; Gouveia, Isabel C.
• Is Part Of: Journal of applied polymer science. Volume 131:Issue 15(2014:Aug. 05)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2014-02-18
• Subjects: Polymers -- Periodicals; Polymerization -- Periodicals; 668.9
• DOI: 10.1002/app.40519
• Languages: English
• ISSNs: 0021-8995

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>The aim of this study was to investigate a method of embedding <sc>l</sc>‐cysteine (<sc>l</sc>‐cys), an antimicrobial agent, between layers of chitosan (CH) and sodium alginate (ALG) onto cotton samples obtained via a layer‐by‐layer electrostatic deposition technique via several embedding methods. The results show that the best way to incorporate <sc>l</sc>‐cys into the layers was the one that used the property of gelling ALG. To monitor the <sc>l</sc>‐cys embedding into the CH/ALG multilayer film, different methods were used: energy‐dispersive X‐ray spectrometry analysis to assess the presence of sulfur on the sample, Ellman's reagent method to analyze <sc>l</sc>‐cys release from the sample, and attenuated total reflectance (ATR) Fourier transform infrared spectroscopy (FTIR) to compare the ATR–FTIR spectra of the pure <sc>l</sc>‐cys and <sc>l</sc>‐cys embedded in the CH/ALG multilayer film to study the interaction between the <sc>l</sc>‐cys and the CH/ALG multilayer films. Functionalized CH/ALG cotton samples were also investigated for their antibacterial properties toward <italic>Staphylococcus aureus</italic> and <italic>Klebsiella pneumonia</italic> with the Japanese Industrial Standard method JIS L 1902:2002, and the results show an enhancement of the antibacterial effect due to the presence of <sc>l</sc>‐cys. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. <bold>2014</bold>, <italic>131</italic>, 40519.</p> </abstract>