Agreement of Serum Spec cPL with the 1, 2‐o‐Dilauryl‐Rac‐Glycero Glutaric Acid‐(6′‐methylresorufin) Ester (DGGR) Lipase Assay and with Pancreatic Ultrasonography in Dogs with Suspected Pancreatitis. (5th March 2014)

• Record Type: Journal Article
• Authors: Kook, P.H.; Kohler, N.; Hartnack, S.; Riond, B.; Reusch, C.E.
• Is Part Of: Journal of veterinary internal medicine. Volume 28:Number 3(2014:May/Jun.)
• Page Start: 863
• Page End: 870
• Publication Date: 2014-03-05
• Subjects: Veterinary medicine -- Periodicals; 636.0896
• DOI: 10.1111/jvim.12334
• Languages: English
• ISSNs: 0891-6640

Abstract

<abstract abstract-type="main" id="jvim12334-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jvim12334-sec-0001" sec-type="section"> <title>Background</title> <p>Spec cPL is the most sensitive and specific test for diagnosing pancreatitis in dogs. Its results have not been compared to those of the 1, 2‐o‐dilauryl‐rac‐glycero‐3‐glutaric acid‐(6′‐methylresorufin) ester (DGGR) lipase assay or those of abdominal ultrasonography.</p> </sec> <sec id="jvim12334-sec-0002" sec-type="section"> <title>Objectives</title> <p>To investigate agreement of Spec cPL with DGGR lipase activity and pancreatic ultrasonography in dogs with suspected pancreatitis.</p> </sec> <sec id="jvim12334-sec-0003" sec-type="section"> <title>Animals</title> <p>One hundred and forty‐two dogs.</p> </sec> <sec id="jvim12334-sec-0004" sec-type="section"> <title>Methods</title> <p>DGGR lipase activity (reference range, 24–108 U/L) and Spec cPL were measured using the same sample. The time interval between ultrasonography and lipase determinations was <24 hours. The agreement of the 2 lipase assays at different cutoffs and the agreement between pancreatic ultrasonography and the 2 tests were assessed using Cohen's kappa coefficient (κ).</p> </sec> <sec id="jvim12334-sec-0005" sec-type="section"> <title>Results</title> <p>DGGR lipase (>108, >216 U/L) and Spec cPL (>200 μg/L) had κ values of 0.79 (95% confidence interval [CI], 0.69–0.9) and 0.70 (CI, 0.58–0.82). DGGR lipase (>108, >216 U/L) and Spec cPL (>400 μg/L) had κ values of 0.55 (CI, 0.43–0.67) and κ of 0.80 (CI, 0.71–0.9). An ultrasonographic diagnosis of pancreatitis and DGGR lipase (>108, >216 U/L) had κ values of 0.29 (CI, 0.14–0.44) and 0.35 (CI, 0.18–0.52). Ultrasonographically diagnosed pancreatitis and Spec cPL (>200, >400 μg/L) had κ values of 0.25 (CI, 0.08–0.41) and 0.27 (CI, 0.09–0.45).</p> </sec> <sec id="jvim12334-sec-0006" sec-type="section"> <title>Conclusions and Clinical Importance</title> <p>Although both lipase assays showed high agreement, agreement between ultrasonography and lipase assays results was only fair. Because lipase results are deemed more accurate, ultrasonography results should be interpreted carefully.</p> </sec> </abstract>