Adiponectin‐impaired adipocyte differentiation negatively regulates fat deposition in chicken. Issue 3 (25th July 2013)

• Record Type: Journal Article
• Authors: Yan, J.; Yang, H.; Gan, L.; Sun, C.
• Is Part Of: Journal of animal physiology and animal nutrition. Volume 98:Issue 3(2014)
• Page Start: 530
• Page End: 537
• Publication Date: 2013-07-25
• Subjects: Animal nutrition -- Periodicals; Feeds -- Periodicals; 636.085
• DOI: 10.1111/jpn.12107
• Languages: English
• ISSNs: 0931-2439

Abstract

<abstract abstract-type="main" id="jpn12107-abs-0001"> <title>Summary</title> <p>Adiponectin is a protein hormone secreted exclusively by adipocytes that plays an important role in the modulation of glucose and lipid metabolism. To investigate the effect of adiponectin on lipid metabolism in chicken, rosiglitazone (agonist of adiponectin) and dexamethasone (inhibitor of adiponectin) were used to treat 23‐day‐old broilers <italic>in vivo</italic>. To verify the functionality of adiponectin on fat deposition, chicken pre‐adipocytes were cultured in the medium containing 10 μg/ml adiponectin. Serum adiponectin and lipids and fat distribution were analysed. Oil Red O staining was used to determine lipid deposition in adipocytes. The expression levels of <italic>adiponectin</italic>, <italic> adiponectin receptors (AdipoR)</italic> and lipid metabolism–related genes in different tissues and pre‐adipocytes were measured using real‐time PCR, and the abundance of lipid metabolism–related proteins was measured by Western blot. Rosiglitazone increased serum adiponectin concentration and the expression levels of <italic>adiponectin</italic> and <italic>adiponectin receptor 1 (AdipoR1)</italic> in tissues and significantly decreased levels of serum lipids and fat deposition. Rosiglitazone significantly increased the expression levels of adipose triglyceride lipase (ATGL) and AdipoR1 and decreased the expression levels of fatty acid synthase (FAS). Dexamethasone had the converse effects compared with rosiglitazone. Oil red O staining results showed a marked decrease in fat deposition in cells treated with adiponectin. In adipocytes, adiponectin could decrease the expression levels of CCAAT/enhancer‐binding protein α (C/EBPα) and FAS and increased the expression levels of ATGL and AdipoR1. These results indicate that adiponectin has a remarkable effect on impairment of adipocyte differentiation, which contributes to the negative regulation of fat deposition in chicken.</p> </abstract>