Effects of β-Glucan on Colon Anastomotic Healing in Rats Given Preoperative Irradiation. (1st June 2014)

• Record Type: Journal Article
• Authors: Seker, Ahmet; Deger, Kamuran Cumhur; Bostanci, Erdal Birol; Ozer, Ilter; Dalgic, Tahsin; Bilgihan, Ayse; Akmansu, Muge; Ekinci, Ozgur; Ercin, Ugur; Akoglu, Musa
• Is Part Of: Journal of investigative surgery. Volume 27:Number 3(2014:Jun.)
• Page Start: 155
• Page End: 162
• Publication Date: 2014-06-01
• Subjects: Surgery -- Research -- Periodicals; Research; Surgery; Surgical Procedures, Operative; 617.075
• DOI: 10.3109/08941939.2013.865820
• Languages: English
• ISSNs: 0894-1939

Abstract

<abstract> <title>ABSTRACT</title> <p> <italic>Background</italic>: Radiation therapy is an essential therapeutic modality in the management of a wide variety of tumors. We aimed to investigate the short-term effects of pelvic irradiation on the healing of colon anastomoses and to determine the potential protective effects of β-glucan in this situation. <italic>Material and Methods</italic>: Sixty Wistar albino rats were randomized into three experimental groups: a control group (<italic>n</italic> = 20), an irradiation (IR) group (<italic>n</italic> = 20), and an irradiation+β-glucan (IR+β-glucan) group (<italic>n</italic> = 20). Only segmental colonic resection and anastomosis were performed on the <italic>control group</italic>. The <italic>IR group</italic> underwent the same surgical procedure as the control group 5 days after pelvic irradiation. In the <italic>IR+β-glucan group</italic>, the same procedure was applied as in the IR group after β-glucan administration. The groups were subdivided into subgroups according to the date of euthanasia (third [<italic>n</italic> = 10] or seventh [<italic>n</italic> = 10] postoperative [PO] day), and anastomotic colonic segments were resected to evaluate bursting pressures and biochemical and histopathological parameters. <italic>Results</italic>: Bursting pressure values were significantly lower in the IR group (<italic>p</italic> < .001). Malondialdehyde (MDA) levels were significantly higher in the IR group, whereas β-glucan significantly decreased MDA levels on the third PO day (<italic>p</italic> < .001). Granulation tissue formation scores were significantly lower in the IR+β-glucan group compared with the control group and the IR group (<italic>p</italic> < .001). <italic>Conclusions</italic>: The results of this study indicate that irradiation has negative effects on the early healing of colon anastomoses. The administration of β-glucan ameliorates these unfavorable effects by altering bursting pressures and biochemical parameters.</p> </abstract>