DNA damage induced by carbon ions (C3+) beam accessed by independent component analysis of infrared spectra. (May 2014)

• Record Type: Journal Article
• Authors: Raposo, Maria; Coelho, Margarida; Gomes, Paulo J.; Vieira, Pedro; Ribeiro, Paulo A.; Mason, Nigel J.; Hunniford, Christopher A.; McCullough, Robert W.
• Is Part Of: International journal of radiation biology. Volume 90:Number 5(2014:May)
• Page Start: 344
• Page End: 350
• Publication Date: 2014-05
• Subjects: Radiation -- Physiological effect -- Periodicals; Radiobiology -- Periodicals; 571.45
• DOI: 10.3109/09553002.2014.892650
• Languages: English
• ISSNs: 0955-3002

Abstract

<abstract> <title>Abstract</title> <p> <italic>Purpose</italic>: To understand the degradation processes and role of secondary species on deoxyribonucleic acid (DNA)-damaged fragments as a result of irradiation with energetic ions.</p> <p> <italic>Materials and methods</italic>: Damage caused to DNA thin films as a result of exposure to 4 keV carbon ions beam was accessed by analyzing the infrared spectra, obtained in situ for different irradiation times, with both bi-dimensional (2D) correlation spectroscopy and independent component analysis (ICA).</p> <p> <italic>Results</italic>: Results indicated that deoxyribose, phosphate and base groups of the DNA molecule were being damaged and new reaction products as oxime and furfural groups are being formed.</p> <p> <italic>Conclusions</italic>: Damage on DNA bases is consistent with the formation of oxime products which react with DNA deoxyribose products forming furfural groups and confirming that DNA damage is caused by direct and indirect processes.</p> </abstract>