Age- and sex-dependent hormonal modulation of the expression of vitamin D receptor and 25-hydroxyvitamin D 1α hydroxylase in human bone cells. Issue 3 (May 2014)

• Record Type: Journal Article
• Authors: Somjen, Dalia; Katzburg, Sara; Hendel, David; Sharon, Orli; Posner, Gary H
• Is Part Of: Expert review of endocrinology & metabolism. Volume 9:Issue 3(2014)
• Page Start: 283
• Page End: 292
• Publication Date: 2014-05
• Subjects: Endocrinology -- Periodicals; Metabolism -- Periodicals; 616.4
• DOI: 10.1586/17446651.2014.899897
• Languages: English
• ISSNs: 1744-6651

Abstract

<abstract> <title> <x xml:space="preserve">Abstract</x> </title> <p>Background: Human cultured osteoblasts from pre-Ob, post-Ob or m-Ob express different mRNAs and respond to different hormones. Aims: To test expression and hormonal modulation of VDR and 1OHase and 1, 25D production in hObs. Methods: hObs obtained from bone explants were prepared, treated and analyzed as before. Results: (i) VDR and 1OHase were expressed in all hObs. (ii) 1, 25D was produced similarly. (iii) Treatment with E₂, DPN (ER agonist), but not PPT (ER agonist) increased VDR. (iv) These hormones increased 1OHase. (v) Vitamin D analog JKF and PTH 1-84 increased similarly mRNAs. (vi) Treatment with E₂, DPN and PPT increased 1, 25D. (vii) JKF and PTH increased similarly 1, 25D. (viii) DNA synthesis and CK were stimulated by all hormones in hObs. Conclusions: Hormonal modulation of VDR and 1OHase and 1, 25D production is important for bone physiology but their correlation, activity and bone physiology is not yet clear.</p> </abstract>