Effect of short‐term (0–72 h) fasting on serum biochemical characteristics in rainbow trout Oncorhynchus mykiss. (22nd April 2013)

• Record Type: Journal Article
• Authors: Hoseini, S. M.; Yousefi, M.; Rajabiesterabadi, H.; Paktinat, M.
• Is Part Of: Journal of applied ichthyology. Volume 30:Number 3(2014:Jun.)
• Page Start: 569
• Page End: 573
• Publication Date: 2013-04-22
• Subjects: Fishes -- Periodicals; Fish culture -- Periodicals; Fisheries -- Periodicals; Ichthyology -- Periodicals; 597
• DOI: 10.1111/jai.12221
• Languages: English
• ISSNs: 0175-8659

Abstract

<abstract abstract-type="main" xml:lang="en" id="jai12221-abs-0001"> <title>Summary</title> <p>An experiment was carried out to investigate the effects of short‐term fasting periods on the serum biochemical characteristics of rainbow trout, <italic>Oncorhynchus mykiss</italic>. For this purpose, fish were fasted 0, 6, 12, 24, 48 and 72 h before blood sampling. Thereafter the serum levels of thyroxine (T<sub>4</sub>), 3, 5, 3′‐triiodothyronine (T<sub>3</sub>), cortisol, glucose, lactate, triglyceride, cholesterol, total protein, albumin, globulin and albumin : globulin ration (A : G) were determined. Results show that serum levels of T<sub>4</sub> (4.60–8.77 ng ml<sup>−1</sup>), T<sub>3</sub> (7.50–13.3 ng ml<sup>−1</sup>), cortisol (7.91–24.5 ng ml<sup>−1</sup>), glucose (18.5–80.1 mg dl<sup>−1</sup>), lactate (12.7–29.6 mg dl<sup>−1</sup>), triglyceride (171–500 mg dl<sup>−1</sup>), and cholesterol (321–535 mg dl<sup>−1</sup>) were significantly affected by the fasting period. However, there were no significant changes in serum total protein (3.03–3.68 g dl<sup>−1</sup>), albumin (1.78–2.01 g dl<sup>−1</sup>), globulin (1.15–1.70 g dl<sup>−1</sup>) or A : G (1.13–1.93) among the fish fasted 0–72 h. Results clearly show the importance of a fasting period on the serum biochemical properties of rainbow trout. According to the results, 24 h fasting is suggested as a pre‐sampling fasting period in rainbow trout to measure serum levels of T<sub>4</sub>, T<sub>3</sub>, cortisol, glucose, lactate, triglyceride, and cholesterol. Potential mechanisms related to the changes in biochemical properties are discussed.</p> </abstract>