In vitro PFOS exposure on immune endpoints in bottlenose dolphins (Tursiops truncatus) and mice. Issue 6 (30th May 2013)

• Record Type: Journal Article
• Authors: Wirth, Jena R.; Peden‐Adams, Margie M.; White, Natasha D.; Bossart, Gregory D.; Fair, Patricia A.
• Is Part Of: Journal of applied toxicology. Volume 34:Issue 6(2014)
• Page Start: 658
• Page End: 666
• Publication Date: 2013-05-30
• Subjects: Toxicology -- Periodicals; Industrial toxicology -- Periodicals; Environmentally induced diseases -- Periodicals; Toxicology -- Periodicals; 615.9005
• DOI: 10.1002/jat.2891
• Languages: English
• ISSNs: 0260-437X

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>Previous studies in our lab have shown that perfluorooctane sulfonate (PFOS) modulates immune function in mice and correlates with many immune parameters in bottlenose dolphins (<italic>Tursiops truncatus</italic>). In this study, bottlenose dolphin peripheral blood leukocytes (PBLs) and adult female B6C3F1 mouse splenocytes were exposed to environmentally relevant PFOS concentrations (0–5 µg ml<sup>–1</sup>) <italic>in vitro</italic>; and natural killer (NK) cell activity and lymphocyte proliferation (T and B cell) were assessed using the parallelogram approach for risk assessment. The objectives were: to corroborate results from the correlative studies in bottlenose dolphins with <italic>in vitro</italic> PFOS exposures; to evaluate the sensitivity of the mouse model as compared with bottlenose dolphins; and to assess risk using the parallelogram approach. In mouse cells, NK cell activity was decreased at <italic>in vitro</italic> doses of 0.01, 0.5, 0.1, 0.5 and 1 µg PFOS ml<sup>–1</sup> and increased at 5 µg ml<sup>–1</sup>. Additionally, B cell proliferation was not altered, but T cell proliferation was decreased at all <italic>in vitro</italic> PFOS exposures. In dolphin cells, NK cell activity and T cell proliferation were not altered by <italic>in vitro</italic> PFOS exposure, but B cell proliferation exhibited a positive association in relation to PFOS dose. Overall, the data indicates that: the <italic>in vitro</italic> exposures of bottlenose dolphin PBLs exhibited results similar to reported correlative fields studies; that mice were generally more sensitive (for these selected endpoints) than were dolphins; and that the parallelogram approach could be used two‐thirds of the time to predict the effects in bottlenose dolphins. Copyright © 2013 John Wiley & Sons, Ltd.</p> </abstract>