Eichhornia crassipes Cleans Wetlands by Enhancing the Nitrogen Removal and Modulating Denitrifying Bacteria Community. Issue 5 (4th October 2013)

• Record Type: Journal Article
• Authors: Yi, Neng; Gao, Yan; Long, Xiao‐hua; Zhang, Zhi‐yong; Guo, Jun‐yao; Shao, Hong‐bo; Zhang, Zhen‐hua; Yan, Shao‐hua
• Is Part Of: Clean. Volume 42:Issue 5(2014:May)
• Page Start: 664
• Page End: 673
• Publication Date: 2013-10-04
• Subjects: Water quality -- Periodicals; Water -- Pollution -- Periodicals; Pollution -- Periodicals; Bioremediation -- Periodicals; Sewage -- Periodicals; Water chemistry -- Periodicals; 333.7205
• DOI: 10.1002/clen.201300211
• Languages: English
• ISSNs: 1863-0650

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="clen201300211-sec-0001" sec-type="section"> <p>The role of floating macrophytes on modulating the microbial nitrogen removal is not well understood. In this study, the cultivation of <italic>Eichhornia crassipes</italic> in eutrophic water may affect the nitrogen (N) fate by modulating the denitrifying bacteria diversity and abundance. The gaseous N losses via denitrification were estimated by <sup>15</sup>N stable isotope tracing and the diversity and abundance of denitrifying genes (<italic>nirS</italic>, <italic>nirK</italic>, and <italic>nosZ</italic>) were investigated by molecular tools. The denaturing gradient gel electrophoresis (DGGE) profiles showed that the diversity of denitrifying genes in the treatments with <italic>E. crassipes</italic> was significantly higher than that in the treatment without <italic>E. crassipes</italic>. The real‐time PCR (qPCR) results showed the trend of denitrifier abundance in the entire system was in the order of N‐ER (nitrate with just root of <italic>E. crassipes</italic>) and A‐ER (ammonia with just root of <italic>E. crassipes</italic>) > N‐R (nitrate with <italic>E. crassipes</italic>) and A‐R (ammonia with <italic>E. crassipes</italic>) > N‐W (nitrate without plant) and A‐W (ammonia without plant). The gaseous <sup>15</sup>N losses via denitrification were significantly and positively related to the abundance of <italic>nirK</italic>, <italic>nirS</italic>, and <italic>nosZ</italic> genes. The results indicated that cultivation of <italic>E. crassipes</italic> in eutrophic water could increase the diversity and abundance of denitrifying bacteria, resulting in more N being removed as gases via denitrification.</p> </sec> </abstract>