Total Synthesis of Homogeneous Variants of Hirudin P6: A Post‐Translationally Modified Anti‐Thrombotic Leech‐Derived Protein1. (11th March 2014)

• Record Type: Journal Article
• Authors: Hsieh, Yves S. Y.; Wijeyewickrema, Lakshmi C.; Wilkinson, Brendan L.; Pike, Robert N.; Payne, Richard J.
• Is Part Of: Angewandte Chemie. Volume 126:Number 15(2014)
• Page Start: 4028
• Page End: 4032
• Publication Date: 2014-03-11
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201310777
• Languages: English
• ISSNs: 0044-8249

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Hirudin P6 is a leech‐derived anti‐thrombotic protein which possesses two post‐translational modifications, <italic>O</italic>‐glycosylation and tyrosine sulfation. In this study we report the ligation‐based synthesis of a library of hirudin P6 proteins possessing homogeneous glycosylation and sulfation modifications. The nature of the modifications incorporated was shown to have a drastic effect on inhibition against both the fibrinogenolytic and amidolytic activities of thrombin and thus highlights a potential means for attenuating the biological activity of the protein.</p> </abstract>