Activation of RANKL‐induced osteoclasts and memory T lymphocytes by Porphyromonas gingivalis is serotype dependant. (May 2014)

• Record Type: Journal Article
• Authors: Vernal, Rolando; Díaz‐Zúñiga, Jaime; Melgar‐Rodríguez, Samanta; Pujol, Myriam; Diaz‐Guerra, Eva; Silva, Augusto; Sanz, Mariano; Garcia‐Sanz, Jose A.
• Is Part Of: Journal of clinical periodontology. Volume 41:Number 5(2014:May)
• Page Start: 451
• Page End: 459
• Publication Date: 2014-05
• Subjects: Periodontics -- Periodicals; 617.6
• DOI: 10.1111/jcpe.12236
• Languages: English
• ISSNs: 0303-6979

Abstract

<abstract abstract-type="main" id="jcpe12236-abs-0001"> <title>Abstract</title> <sec id="jcpe12236-sec-0001" sec-type="section"> <title>Aim</title> <p>Destructive periodontitis is associated with a Th1–Th17 immune response and activation of RANKL‐induced osteoclasts. In addition, <italic>Porphyromonas gingivalis</italic> K1 and K2 serotypes induce a strong Th1–Th17 response. This study aimed to investigate whether these <italic>P. gingivalis</italic> serotypes induce higher osteoclasts activation, by increased Th17‐associated RANKL production, and an antigen‐specific memory T‐lymphocyte response.</p> </sec> <sec id="jcpe12236-sec-0002" sec-type="section"> <title>Material and Methods</title> <p>The RANKL production and TRAP<sup>+</sup> osteoclast induction were quantified on naïve T lymphocytes stimulated with dendritic cells primed with the <italic>P</italic>. <italic>gingivalis</italic> serotypes. The T‐bet, GATA‐3, RORC2 and Foxp3 expression was correlated with RANKL production. The frequency of proliferating memory T lymphocytes in response to <italic>P. gingivalis</italic> serotypes was determined in both periodontitis and healthy subjects.</p> </sec> <sec id="jcpe12236-sec-0003" sec-type="section"> <title>Results</title> <p>T lymphocytes stimulated by K1 or K2‐primed dendritic cells elicited higher levels of RANKL and TRAP<sup>+</sup> osteoclasts than cells stimulated with the other serotypes. RANKL positively correlated with RORC2. Whereas periodontitis patients had a higher frequency of memory T lymphocytes responding to K1 or K2, healthy subjects had a higher frequency of memory T lymphocytes responding to K4 or K<sup>−</sup>.</p> </sec> <sec id="jcpe12236-sec-0004" sec-type="section"> <title>Conclusions</title> <p> <italic>P. gingivalis</italic> serotypes K1 and K2, but not others, are associated with an increased production of the osteoclastogenesis‐related factor RANKL. This important information suggests that these serotypes could elicit a greater bone resorption in vivo and have a role in the periodontitis pathogenesis.</p> </sec> </abstract>