Subgingival microbiota of Sri Lankan tea labourers naïve to oral hygiene measures. (26th February 2014)

• Record Type: Journal Article
• Authors: Zhuang, Long‐Fei; Watt, Rory M.; Steiner, Sarah; Lang‐Hua, Bich Hue; Wang, Ren; Ramseier, Christoph A.; Lang, Niklaus P.
• Is Part Of: Journal of clinical periodontology. Volume 41:Number 5(2014:May)
• Page Start: 433
• Page End: 441
• Publication Date: 2014-02-26
• Subjects: Periodontics -- Periodicals; 617.6
• DOI: 10.1111/jcpe.12230
• Languages: English
• ISSNs: 0303-6979

Abstract

<abstract abstract-type="main" id="jcpe12230-abs-0001"> <title>Abstract</title> <sec id="jcpe12230-sec-0001" sec-type="section"> <title>Aim</title> <p>To characterize the subgingival microbiota within a cohort of adult males (<italic>n</italic> = 32) naïve to oral hygiene practices, and to compare the composition of bacterial taxa present in periodontal sites with various probing depths.</p> </sec> <sec id="jcpe12230-sec-0002" sec-type="section"> <title>Material and Methods</title> <p>Subgingival plaque samples were collected from single shallow pocket [pocket probing depth (PPD)≤3 mm] and deep pocket (PPD≥6 mm) sites from each subject. A polymerase chain reaction based strategy was used to construct a clone library of 16S ribosomal RNA (rRNA) genes for each site. The sequences of ca. 30–60 plasmid clones were determined for each site to identify resident taxa. Microbial composition was compared using a variety of statistical and bioinformatics approaches.</p> </sec> <sec id="jcpe12230-sec-0003" sec-type="section"> <title>Results</title> <p>A total of 1887 cloned 16S rRNA gene sequences were analysed, which were assigned to 318 operational taxonomic units (98% identity cut‐off). The subgingival microbiota was dominated by <italic>Firmicutes</italic> (69.8%), <italic>Proteobacteria</italic> (16.3%), and <italic>Fusobacteria</italic> (8.0%). The overall composition of microbial communities in shallow sites was significantly different from those within deep sites (∫‐Libshuff, <italic>p</italic> < 0.001).</p> </sec> <sec id="jcpe12230-sec-0004" sec-type="section"> <title>Conclusions</title> <p>A taxonomically diverse subgingival microbiota was present within this cohort; however, the structures of the microbial communities present in the respective subjects exhibited limited variation. Deep and shallow sites contained notably different microbial compositions, but this was not correlated with the rate of periodontal progression.</p> </sec> </abstract>