Initiation of the flexirubin biosynthesis in Chitinophaga pinensis. Issue 3 (28th January 2014)

• Record Type: Journal Article
• Authors: Schöner, Tim A.; Fuchs, Sebastian W.; Schönau, Christian; Bode, Helge B.
• Is Part Of: Microbial biotechnology. Volume 7:Issue 3(2014:May)
• Page Start: 232
• Page End: 241
• Publication Date: 2014-01-28
• Subjects: Microbial biotechnology -- Periodicals; Biotechnology; Microbiology; 660.62
• DOI: 10.1111/1751-7915.12110
• Languages: English
• ISSNs: 1751-7915

Abstract

<abstract abstract-type="main"> <title>Summary</title> <p>Bacteria from the Bacteroidetes phylum are known producers of the chemotaxonomic relevant flexirubins. These orange pigments comprise a non‐isoprenoid aryl‐polyene carboxylic acid esterified with a dialkylresorcinol. Herein, we report a gene cluster from <italic>C</italic><italic>hitinophaga pinensis</italic> encoding the biosynthesis of the polyene moiety and the biochemical characterization of a tyrosine ammonia‐lyase and a 4‐coumarate‐CoA ligase responsible for the initiation of the polyene biosynthesis. Additionally, the flexirubin of <italic>C</italic><italic>. pinensis</italic> was characterized by a combination of feeding experiments, high‐performance liquid chromatography tandem mass spectrometry and matrix‐assisted laser desorption/ionization mass spectrometry.</p> </abstract>