Effects of Hg(II) Exposure on MAPK Phosphorylation and Antioxidant System in D. melanogaster. Issue 6 (14th June 2012)

• Record Type: Journal Article
• Authors: Paula, M. T.; Zemolin, A. P.; Vargas, A. P.; Golombieski, R. M.; Loreto, E. L. S.; Saidelles, A. P.; Picoloto, R. S.; Flores, E. M. M.; Pereira, A. B.; Rocha, J. B. T.; Merritt, T. J. S.; Franco, J. L.; Posser, T.
• Is Part Of: Environmental toxicology. Volume 29:Issue 6(2014:Jun.)
• Page Start: 621
• Page End: 630
• Publication Date: 2012-06-14
• Subjects: Water quality bioassay -- Periodicals; Water -- Pollution -- Toxicology -- Periodicals; Microbiological assay -- Periodicals; Toxicity testing -- Periodicals; Environmental toxicology -- Periodicals; Environmental Pollution -- Periodicals; Environmental Pollutants -- Periodicals; Environmental Monitoring -- Periodicals; Écotoxicologie -- Périodiques; Pollution -- Périodiques; 615.902
• DOI: 10.1002/tox.21788
• Languages: English
• ISSNs: 1520-4081

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The heavy metal mercury is a known toxin, but while the mechanisms involved in mercury toxicity have been well demonstrated in vertebrates, little is known about toxicological effects of this metal in invertebrates. Here, we present the results of our study investigating the effects associated with exposure of fruit fly <italic>Drosophila melanogaster</italic> to inorganic mercury (HgCl<sub>2</sub>). We quantify survival and locomotor performance as well as a variety of biochemical parameters including antioxidant status, MAPK phosphorylation and gene expression following mercury treatment. Our results demonstrate that exposure to Hg(II) through diet induced mortality and affected locomotor performance as evaluated by negative geotaxis, in <italic>D. melanogaster</italic>. We also saw a significant impact on the antioxidant system including an inhibition of acetylcholinesterase (Ache), glutathione S‐transferase (GST) and superoxide dismutase (SOD) activities. We found no significant alteration in the levels of mRNA of antioxidant enzymes or NRF‐2 transcriptional factor, but did detect a significant up regulation of the <italic>HSP83</italic> gene. Mercury exposure also induced the phosphorylation of JNK and ERK, without altering p38<sup>MAPK</sup> and the concentration of these kinases. In parallel, Hg(II) induced PARP cleavage in a 89 kDa fragment, suggesting the triggering of apoptotic cell death in response to the treatment. Taken together, this data clarifies and extends our understanding of the molecular mechanisms mediating Hg(II) toxicity in an invertebrate model. © 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 621–630, 2014.</p> </abstract>