Cover Picture: RNA‐Directed Amino Acid Coupling as a Model Reaction for Primitive Coded Translation (ChemBioChem 6/2014). Issue 6 (14th April 2014)

• Record Type: Journal Article
• Authors: Harada, Kazuo; Aoyama, Shoko; Matsugami, Akimasa; Kumar, Penmetcha K. R.; Katahira, Masato; Kato, Nobuo; Ohkanda, Junko
• Is Part Of: Chembiochem. Volume 15:Issue 6(2014)
• Page Start: 769
• Page End: 769
• Publication Date: 2014-04-14
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201490016
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>The cover picture shows</bold> the NMR structure of the Tat aptamer RNA (the ribose–phosphate backbone of the RNA is shown as a blue ribbon representation) in complex with two argininamide molecules (upper right) and details of the RNA–amino acid interactions (lower left). On <ext-link ext-link-type="doi" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink">p. 794 ff., </ext-link> K. Harada et al. reveal the NMR structure of this complex. The U bases (U7 and U23) in the bulges form U:A:U base triplets accompanied by coaxial stacking of the stems, and this forms a binding pocket for the two argininamide molecules (red). The guanidinium side chain can thus donate hydrogen bonds to the O6 and N7 groups on the G bases (G9 and G25) in the major groove. The formulae on the lower left show how the guanidinium groups of the arginines are hydrogen bonded to the Hoogsteen sites of G9 and G25, thus positioning the two amino acids for peptide bond formation using an activating agent.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg56g493g6" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>