Cord blood graft composition impacts the clinical outcome of allogeneic stem cell transplantation. Issue 2 (24th January 2014)

• Record Type: Journal Article
• Authors: Wikell, H.; Ponandai‐Srinivasan, S.; Mattsson, J.; Gertow, J.; Uhlin, M.
• Is Part Of: Transplant infectious disease. Volume 16:Issue 2(2014)
• Page Start: 203
• Page End: 212
• Publication Date: 2014-01-24
• Subjects: Transplantation of organs, tissues, etc -- Complications -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 617.01
• DOI: 10.1111/tid.12182
• Languages: English
• ISSNs: 1398-2273

Abstract

<abstract abstract-type="main" id="tid12182-abs-0001"> <title>Abstract</title> <sec id="tid12182-sec-0001" sec-type="section"> <title>Introduction</title> <p>Despite routine use of umbilical cord blood (CB) grafts as stem cell source for allogeneic stem cell transplantations, much remains unknown regarding their cell composition and correlation with clinical outcome.</p> </sec> <sec id="tid12182-sec-0002" sec-type="section"> <title>Methods</title> <p>We analyzed material from 30 CB units used for allogeneic hematopoietic stem cell transplantation by multicolor flow cytometry. Phenotypic data were correlated with various clinical outcomes such as survival, graft‐versus‐host disease (GVHD), relapse, rejection, viral reactivation, and bacteremia.</p> </sec> <sec id="tid12182-sec-0003" sec-type="section"> <title>Results</title> <p>We found that above‐median frequencies of CD69+ T cells, naïve CD8+ T cells, and CD127+ B cells in the CB graft were each associated with significantly improved patient survival. Moreover, a statistically significant correlation was seen between higher levels of CD94+ T cells and herpes simplex virus and varicella zoster virus reactivation post transplantation. A similar correlation was seen for the frequency of CD95+ cells in total CD3+, as well as CD4+ and CD8+ T‐cell subsets, and viral reactivation. Finally, a higher frequency of naïve CD8+ T cells was associated with the incidence of acute GVHD.</p> </sec> <sec id="tid12182-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Our study highlights the importance of further exploration of graft composition before CB transplantation as a tool for risk prediction.</p> </sec> </abstract>