Isolation of Treponema and Tannerella spp. from equine odontoclastic tooth resorption and hypercementosis related periodontal disease. (17th September 2013)

• Record Type: Journal Article
• Authors: Sykora, S.; Pieber, K.; Simhofer, H.; Hackl, V.; Brodesser, D.; Brandt, S.
• Is Part Of: Equine veterinary journal. Volume 46:Number 3(2014:May)
• Page Start: 358
• Page End: 363
• Publication Date: 2013-09-17
• Subjects: Horses -- Diseases -- Periodicals; 636.108905
• DOI: 10.1111/evj.12115
• Languages: English
• ISSNs: 0425-1644

Abstract

<abstract abstract-type="main"> <title>Summary</title> <sec id="evj12115-sec-1001" sec-type="section"> <title>Reasons for performing study</title> <p>Red complex bacteria, i.e. <italic>Porphyromonas gingivalis</italic>, <italic>Treponema denticola</italic> and <italic>Tannerella forsythia, </italic> are involved in the onset and progression of periodontal disease in man, yet seldom inhabit the oral cavity of healthy individuals. Periodontal disease is also encountered in horses, with equine odontoclastic tooth resorption and hypercementosis (EOTRH) constituting a particular form of disease. However, only little is known about the oral microbiome of healthy and periodontitis‐affected equids.</p> </sec> <sec id="evj12115-sec-1002" sec-type="section"> <title>Objective</title> <p>We aimed to test the hypothesis that red complex bacteria are also associated with EOTRH‐related periodontal disease.</p> </sec> <sec id="evj12115-sec-1003" sec-type="section"> <title>Study design</title> <p>Controlled cross‐sectional study.</p> </sec> <sec id="evj12115-sec-1004" sec-type="section"> <title>Methods</title> <p>We screened DNA purified from crevicular fluid derived from 23 EOTRH‐affected and 21 disease‐free horses for the presence of <italic>Treponema</italic> spp., <italic>Tannerella</italic> spp. and <italic>Porphyromonas gingivalis</italic> DNA by polymerase chain reaction. Subsequently, amplified DNA was bidirectionally sequenced and identified via BLAST analysis.</p> </sec> <sec id="evj12115-sec-1005" sec-type="section"> <title>Results</title> <p> <italic>Treponema</italic> and/or <italic>Tannerella</italic> DNA was detected in 100% of periodontitis‐related samples and in 52.2% of DNA derived from healthy horses. Twenty‐six amplicon sequences were 98–100% homologous to published bacterial sequences, which mostly corresponded to <italic>Treponema pectinovorum</italic>, oral <italic>Treponema</italic> clones JU025 and OMZ 840, and <italic>Tannerella forsythia</italic>. <italic>P. gingivalis</italic> DNA was only found in 3 EOTRH‐related samples. Forty‐three amplicon sequences revealed weaker homologies ranging between 80% and 97% to known <italic>Treponema</italic> or <italic>Tannerella</italic> strains, partly because of their heterogeneity, partly because they obviously represented so far unknown types.</p> </sec> <sec id="evj12115-sec-1006" sec-type="section"> <title>Conclusions</title> <p>This is the first report in which known and novel <italic>Treponema</italic> and <italic>Tannerella</italic> spp. were isolated in association with EOTRH‐related periodontal disease.</p> </sec> </abstract>