Temporal expression of agrB, cidA, and alsS in the early development of Staphylococcus aureus UAMS‐1 biofilm formation and the structural role of extracellular DNA and carbohydrates. Issue 3 (11th March 2014)

• Record Type: Journal Article
• Authors: Grande, Rossella; Nistico, Laura; Sambanthamoorthy, Karthik; Longwell, Mark; Iannitelli, Antonio; Cellini, Luigina; Di, Antonio; Hall Stoodley, Luanne; Stoodley, Paul
• Is Part Of: Pathogens and disease. Volume 70:Issue 3(2014:Apr.)
• Page Start: 414
• Page End: 422
• Publication Date: 2014-03-11
• Subjects: Medical microbiology -- Periodicals; Pathogenic microorganisms -- Periodicals; Communicable diseases -- Microbiology -- Periodicals; Communicable diseases -- Pathogenesis -- Periodicals; Host-parasite relationships -- Periodicals; Systems biology -- Periodicals; 616.904105
• DOI: 10.1111/2049-632X.12158
• Languages: English
• ISSNs: 2049-632X

Abstract

<abstract abstract-type="main" id="fim12158-abs-0001"> <title>Abstract</title> <p>Extracellular DNA (eDNA) is an important component of the extracellular polymeric substance matrix and is important in the establishment and persistence of <italic>Staphylococcus aureus </italic>UAMS‐1 biofilms. The aim of the study was to determine the temporal expression of genes involved in early biofilm formation and eDNA production. We used qPCR to investigate expression of <italic>agrB</italic>, which is associated with secreted virulence factors and biofilm dispersal, <italic>cidA</italic>, which is associated with biofilm adherence and genomic DNA release, and <italic>alsS, </italic> which is associated with cell lysis, eDNA release and acid tolerance. The contribution of eDNA to the stability of the biofilm matrix was assessed by digesting with DNase I (Pulmozyme) and quantifying structure by confocal microscopy and <sc>comstat</sc> image analysis. <italic>AgrB</italic> expression initially increased at 24 h but then dramatically decreased at 72 h in an inverse relationship to biomass, supporting its role in regulating biofilm dispersal. <italic>cidA</italic> and <italic>alsS</italic> expression steadily increased over 72 h, suggesting that eDNA was an important component of early biofilm development. DNase I had no effect on biomass, but did cause the biofilms to become more heterogeneous. Carbohydrates in the matrix appeared to play an important role in structural stability.</p> </abstract>