Importance of biofilm formation and dipeptidyl peptidase IV for the pathogenicity of clinical Porphyromonas gingivalis isolates. Issue 3 (11th March 2014)

• Record Type: Journal Article
• Authors: Clais, Sofie; Boulet, Gaëlle; Kerstens, Monique; Horemans, Tessa; Teughels, Wim; Quirynen, Marc; Lanckacker, Ellen; De, Ingrid; Lambeir, Anne‐Marie; Delputte, Peter; Maes, Louis; Cos, Paul
• Is Part Of: Pathogens and disease. Volume 70:Issue 3(2014:Apr.)
• Page Start: 408
• Page End: 413
• Publication Date: 2014-03-11
• Subjects: Medical microbiology -- Periodicals; Pathogenic microorganisms -- Periodicals; Communicable diseases -- Microbiology -- Periodicals; Communicable diseases -- Pathogenesis -- Periodicals; Host-parasite relationships -- Periodicals; Systems biology -- Periodicals; 616.904105
• DOI: 10.1111/2049-632X.12156
• Languages: English
• ISSNs: 2049-632X

Abstract

<abstract abstract-type="main" id="fim12156-abs-0001"> <title>Abstract</title> <p>The ability of <italic>Porphyromonas gingivalis</italic> to cause adult periodontitis is determined by its arsenal of virulence factors. Here, we investigated the importance of biofilm formation and bacterial dipeptidyl peptidase IV (DPPIV) for the pathogenicity of clinical <italic>P. gingivalis</italic> isolates. In our study, the isolates with biofilm‐forming capacity also showed high DPPIV activity <italic>in vitro</italic>. Moreover, DPPIV activity increased in <italic>P. gingivalis</italic> biofilms compared to planktonic cells. In a murine subcutaneous abscess model, the biofilm‐forming isolates with high DPPIV activity proved to be pathogenic, while the nonbiofilm formers with low DPPIV activity did not induce abscesses. The biofilm‐forming ATCC 33277 strain with low DPPIV activity was not pathogenic in mice either. Our results suggest that biofilm formation and DPPIV activity contribute to the pathogenic potential of <italic>P. gingivalis</italic>. Furthermore, we show that biofilm formation may enhance <italic>P. gingivalis</italic> virulence through an increased DPPIV activity. Because of their importance for bacterial colonization and growth, biofilm formation and DPPIV activity could present interesting therapeutic targets to tackle periodontitis.</p> </abstract>