Multiple effects of Escherichia coli Nissle 1917 on growth, biofilm formation, and inflammation cytokines profile of Clostridium perfringens type A strain CP4. Issue 3 (11th March 2014)

• Record Type: Journal Article
• Authors: Jiang, Yanlong; Kong, Qingke; Roland, Kenneth L.; Wolf, Amanda; Curtiss, Roy
• Is Part Of: Pathogens and disease. Volume 70:Issue 3(2014:Apr.)
• Page Start: 390
• Page End: 400
• Publication Date: 2014-03-11
• Subjects: Medical microbiology -- Periodicals; Pathogenic microorganisms -- Periodicals; Communicable diseases -- Microbiology -- Periodicals; Communicable diseases -- Pathogenesis -- Periodicals; Host-parasite relationships -- Periodicals; Systems biology -- Periodicals; 616.904105
• DOI: 10.1111/2049-632X.12153
• Languages: English
• ISSNs: 2049-632X

Abstract

<abstract abstract-type="main" id="fim12153-abs-0001"> <title>Abstract</title> <p> <italic>Clostridium perfringens</italic> is an important Gram‐positive pathogen responsible for food poisoning, necrotic enteritis, gas gangrene, and even death. <italic>Escherichia coli</italic> Nissle 1917 (EcN) is a well‐characterized probiotic strain with demonstrated benefits. In this study, we evaluated the effects of EcN on growth, toxin production, biofilm formation, and inflammatory cytokine responses of <italic>C. perfringens</italic>. <italic>In vitro</italic> co‐culture experiments demonstrated that EcN inhibited growth, gas production, and toxin production (α‐toxin and NetB) of <italic>C. perfringens</italic> in a dose‐dependent manner. The growth inhibition effect was not observed when <italic>C. perfringens</italic> was incubated with EcN cell‐free supernatants (CFS<sub>E</sub>), suggesting that growth inhibition was caused by nutrition competition during co‐incubation. <italic>In vitro</italic> studies demonstrated that pre‐incubation with EcN did not inhibit <italic>C. perfringens</italic> attachment to Caco‐2 cells, but did reduce <italic>C. perfringens</italic> total number, toxin production, and cytotoxicity after 24 h. The similar growth inhibition results were also observed during the formation of <italic>C. perfringens</italic> biofilm. Finally, pre‐incubation of EcN with RAW264.7 cells significantly decreased the production of inflammatory cytokines caused by the introduction of <italic>C. perfringens</italic>. Our results indicate that EcN can inhibit many of the pathological effects of <italic>C. perfringens in vitro</italic> conditions.</p> </abstract>