Isolation of viable but nonculturable Vibrio cholerae O1 from environmental water samples in Kolkata, India, in a culturable state. Issue 2 (18th February 2014)

• Record Type: Journal Article
• Authors: Senoh, Mitsutoshi; Ghosh‐Banerjee, Jayeeta; Mizuno, Tamaki; Shinoda, Sumio; Miyoshi, Shin‐ichi; Hamabata, Takashi; Nair, G. Balakrish; Takeda, Yoshifumi
• Is Part Of: MicrobiologyOpen. Volume 3:Issue 2(2014:Apr.)
• Page Start: 239
• Page End: 246
• Publication Date: 2014-02-18
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1002/mbo3.164
• Languages: English
• ISSNs: 2045-8827

Abstract

<abstract abstract-type="main" id="mbo3164-abs-0001"> <title>Abstract</title> <p>Previously, we reported that viable but nonculturable (VBNC) <italic>Vibrio cholerae</italic> was converted into a culturable state by coculture with several eukaryotic cell lines including HT‐29 cells. In this study, we found that a factor converting VBNC <italic>V. cholerae</italic> into a culturable state (FCVC) existed in cell extracts of eukaryotic cells. FCVC was nondialyzable, proteinase K‐sensitive, and stable to heating at &lt;60°C for 5 min. We prepared thiosulfate citrate bile salts sucrose (TCBS) plates with FCVC (F‐TCBS plates). After confirming that VBNC <italic>V. cholerae</italic> O1 and O139 formed typical yellow colonies on F‐TCBS plates, we tried to isolate cholera toxin gene‐positive VBNC <italic>V. cholerae</italic> from environmental water samples collected in urban slum areas of Kolkata, India and succeeded in isolating <italic>V. cholerae</italic> O1 El Tor variant strains harboring a gene for the cholera toxin. The possible importance of VBNC <italic>V. cholerae</italic> O1 as a source of cholera outbreaks is discussed.</p> </abstract>