It's in your blood: spectral biomarker candidates for urinary bladder cancer from automated FTIR spectroscopy. Issue 3 (7th January 2014)

• Record Type: Journal Article
• Authors: Ollesch, Julian; Heinze, Margot; Heise, H. Michael; Behrens, Thomas; Brüning, Thomas; Gerwert, Klaus
• Is Part Of: Journal of biophotonics. Volume 7:Issue 3/4(2014)
• Page Start: 210
• Page End: 221
• Publication Date: 2014-01-07
• Subjects: Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201300163
• Languages: English
• ISSNs: 1864-063X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Blood samples of urinary bladder cancer (UBC) patients and patients with urinary tract infection were analysed with advanced automated high throughput Fourier transform infrared (HT‐FTIR)‐spectroscopy. Thin dried film samples were robotically prepared on multi‐well titer plates (MTP) for absorbance measurements in transmission mode. Within the absorbance, 1st and 2nd derivative spectra of serum and two plasma preparations, discriminative patterns were identified and validated using bioinformatic tools. The optimal spectral resolution for data acquisition was determined. An accurate discrimination of the patient groups was achieved with three different independent spectral variable sets. The HT‐FTIR blood test may support future clinical diagnostics. (© 2014 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)</p> </abstract>