Dynamic fluorescent imaging with indocyanine green for monitoring the therapeutic effects of photoimmunotherapy. (19th March 2014)

• Record Type: Journal Article
• Authors: Ali, Towhid; Nakajima, Takahito; Sano, Kohei; Sato, Kazuhide; Choyke, Peter L.; Kobayashi, Hisataka
• Is Part Of: Contrast media & molecular imaging. Volume 9:Number 4(2014:Jul./Aug.)
• Page Start: 276
• Page End: 282
• Publication Date: 2014-03-19
• Subjects: Diagnostic imaging -- Periodicals; Magnetic resonance imaging -- Periodicals; Contrast media (Diagnostic imaging) -- Periodicals; Contrast Media -- Periodicals; Diagnostic Imaging -- Periodicals; Substances de contraste -- Périodiques; Diagnostics moléculaires -- Périodiques; Imagerie médicale; Substance de contraste; Périodique électronique (Descripteur de forme); Ressource Internet (Descripteur de forme); 616.0754
• DOI: 10.1002/cmmi.1570
• Languages: English
• ISSNs: 1555-4309

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>A new type of monoclonal antibody (mAb)‐based, highly specific phototherapy (photoimmunotherapy; PIT) that uses a near‐infrared (NIR) phthalocyanine dye, IRDye700DX (IR700) conjugated with an mAb, has recently been described. NIR light exposure leads to immediate, target‐selective necrotic cell death. However, tumor shrinkage takes several days to occur, making it difficult to detect earlier changes in the tumor. In this study, Panitumumab targeting the epidermal growth factor receptor (EGFR1) conjugated to IR700 was used to treat EGFR‐expressing A431 tumor cells and <italic>in vivo</italic> xenografts. PIT was performed at varying doses of NIR light (10, 30, 50 and 100 J cm<sup>−2</sup>) in xenograft tumors in mice. Indocyanine green (ICG) dynamic imaging was evaluated for monitoring cytotoxic effects for the first hour after PIT. Our results demonstrated a statistical difference (<italic>p</italic> < 0.05) in ICG intensity between control and PIT treated tumors in the higher light exposure groups (50 J cm<sup>−2</sup>: 2.94 ± 0.35 vs 5.22 ± 0.92, <italic>p</italic> = 0.02; and 100 J cm<sup>−2</sup>: 3.56 ± 0.96 vs 5.71 ± 1.43, <italic>p</italic> = 0.008) as early as 20 min post ICG injection. However, no significant difference (<italic>p</italic> > 0.05) in ICG intensity between control and PIT treated tumors was evident in the lower light exposure group at any time points up to 60 min (10 J cm<sup>−2</sup>: 1.92 ± 0.49 vs 1.71 ± 0.3, <italic>p</italic> = 0.44; and 30 J cm<sup>−2</sup>: 1.57 ± 0.35 vs 2.75 ± 0.59, <italic>p</italic> = 0.07). Similarly, the retention index (background to corrected uptake ratio of ICG) varied with light exposure. In conclusion, ICG may serve as a potential indicator of acute cytotoxic effects of mAb‐IR700‐induced PIT even before morphological changes can be seen in targeted tumors. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.</p> </abstract>