Effect of Dietary N‐acyl Homoserin Lactonase on the Immune Response and the Gut Microbiota of Zebrafish, Danio rerio, Infected with Aeromonas hydrophila. Issue 2 (April 2014)

• Record Type: Journal Article
• Authors: Cao, Yanan; Liu, Yuchun; Mao, Wei; Chen, Ruidong; He, Suxu; Gao, Xiuhua; Zhou, Zhigang; Yao, Bin
• Is Part Of: Journal of the World Aquaculture Society. Volume 45:Issue 2(2014)
• Page Start: 149
• Page End: 162
• Publication Date: 2014-04
• Subjects: Aquaculture -- Periodicals; 639.8
• DOI: 10.1111/jwas.12105
• Languages: English
• ISSNs: 0893-8849

Abstract

<abstract abstract-type="main" id="jwas12105-abs-0001"> <title>Abstract</title> <p id="jwas12105-para-0001"> <bold>Quorum sensing interference is a novel strategy in bacterial disease control. Degradation of quorum sensing signal molecules, such as <italic>N</italic>‐acyl homoserine lactones (AHLs), by enzymes is a potential interfering method. In this study, the <italic>in vivo</italic> effect of an <italic>N</italic>‐acyl homoserine lactonase from <italic>Bacillus</italic> sp. B546, AiiA<sub>B546</sub>, on the protection of zebrafish, <italic>Danio rerio</italic>, against <italic>Aeromonas hydrophila</italic> infection was first investigated by co‐feeding. The short‐term immune responses and the long‐term effects on the gut microbiota of zebrafish were analyzed by quantitative polymerase chain reaction (qPCR) and denaturing gradient gel electrophoresis (DGGE), respectively. The results showed that the expression levels of some anti‐infectious immunity‐related factors, including interleukin 10 (IL‐10), toll‐like receptor 5b (TLR5b), and inducible nitric oxide synthase 2a (iNOS2a) that were related to <italic>A</italic>. <italic>hydrophila</italic> virulent factors under regulation of quorum sensing, were more susceptible to the addition of AiiA<sub>B546</sub>. It indicated that AiiA<sub>B546</sub> may attenuate the infection of <italic>A</italic>. <italic>hydrophila</italic> by interfering with quorum sensing. <italic>A</italic>. <italic>hydrophila</italic> was not detected in the enzyme co‐feeding diet group, which might be due to its inability to adhere to the gut with enzyme interference. This study revealed that AiiA<sub>B546</sub> as a feed additive might represent a novel biocontrol agent in aquaculture; however, the topic merits further investigations using fish species of commercial interest.</bold> </p> </abstract>