CDNA cloning and expression characterization of serum transferrin gene from oriental weatherfish Misgurnus anguillicaudatus. Issue 4 (12th February 2014)

• Record Type: Journal Article
• Authors: Chen, J. H.; Wang, C. H.; Li, Y. L.; Wang, H. M.; Zhang, X. J.; Yan, B. L.
• Is Part Of: Journal of fish biology. Volume 84:Issue 4(2014)
• Page Start: 885
• Page End: 896
• Publication Date: 2014-02-12
• Subjects: Fishes -- Periodicals; Fishes -- Great Britain -- Periodicals; 597
• DOI: 10.1111/jfb.12307
• Languages: English
• ISSNs: 0022-1112

Abstract

<abstract abstract-type="main" id="jfb12307-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p id="jfb12307-para-0001">In this study, the cDNA coding serum transferrin (<italic>stf</italic>) of <italic>Misgurnus anguillicaudatus</italic> (<italic>mastf</italic>) was cloned. <italic>mastf</italic> cDNA is composed of 2326 bp with a 2007 bp open reading frame encoding 668 amino acids. The deduced Mastf protein consists of a signal peptide, two lobes (N and C‐lobes) and signature motifs of transferrin (Tf) family. The results of tissue distribution indicated that <italic>mastf</italic> mRNA was predominantly expressed in the liver. The results indicate that the <italic>mastf</italic> expression increased significantly in liver, blood, spleen and head kidney after the challenge with <italic>Aeromonas sobria</italic>, acting as a positive acute protein, suggesting that <italic>mastf</italic> is related to the immune response. The cloning and expression analysis of <italic>mastf</italic> further demonstrates the evolutionary conservation of Stf and immune function in vertebrates.</p> </abstract>