Evaluation of Serum Phosphorylated Neurofilament Subunit NF‐H as a Prognostic Biomarker in Dogs With Thoracolumbar Intervertebral Disc Herniation. Issue 3 (27th January 2014)

• Record Type: Journal Article
• Authors: Nishida, Hidetaka; Nakayama, Masanari; Tanaka, Hiroshi; Kamishina, Hiroaki; Izawa, Takeshi; Hatoya, Shingo; Sugiura, Kikuya; Suzuki, Yoshihisa; Ide, Chizuka; Inaba, Toshio
• Is Part Of: Veterinary surgery. Volume 43:Issue 3(2014)
• Page Start: 289
• Page End: 293
• Publication Date: 2014-01-27
• Subjects: Veterinary surgery -- Periodicals; Veterinary Medicine -- Periodicals; Surgery -- Periodicals; Societies, Medical -- Periodicals; 636.0897
• DOI: 10.1111/j.1532-950X.2014.12144.x
• Languages: English
• ISSNs: 0161-3499

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="vsu12144-sec-0001" sec-type="section"> <title>Objective</title> <p>To investigate whether pNF‐H is a prognostic biomarker of spinal cord injury (SCI) in paraplegic dogs with thoracolumbar intervertebral disc herniation (IVDH).</p> </sec> <sec id="vsu12144-sec-0002" sec-type="section"> <title>Study Design</title> <p>Prospective, case‐control clinical study</p> </sec> <sec id="vsu12144-sec-0003" sec-type="section"> <title>Animals</title> <p>Dogs (n = 60) with SCI from IVDH and 6 healthy dogs.</p> </sec> <sec id="vsu12144-sec-0004" sec-type="section"> <title>Methods</title> <p>Serum from 60 thoracolumbar IVDH dogs (Grade 4: 22 dogs; Grade 5: 38 dogs) collected 1–3 days after injury, and 6 control dogs, was analyzed using enzyme‐linked immunosorbent assay (ELISA) against a phosphorylated form of the high‐molecular‐weight neurofilament subunit NF‐H (pNF‐H). Serum pNF‐H levels were compared between different IVDH grades and their prognostic value was investigated.</p> </sec> <sec id="vsu12144-sec-0005" sec-type="section"> <title>Results</title> <p>pNF‐H levels were significantly greater in Grade 5 than Grade 4 dogs. There were significant differences in pNF‐H levels between dogs that regained voluntarily ambulation and those that did not. All 8 dogs that had high pNF‐H levels 1–3 days after injury did not regain the ability to walk after surgery.</p> </sec> <sec id="vsu12144-sec-0006" sec-type="section"> <title>Conclusions</title> <p>Serum pNF‐H levels might be a biomarker for predicting prognosis of canine SCI.</p> </sec> </abstract>