Effect of d‐fagomine on excreted enterobacteria and weight gain in rats fed a high‐fat high‐sucrose diet. (4th December 2013)

• Record Type: Journal Article
• Authors: Ramos‐Romero, Sara; Molinar‐Toribio, Eunice; Gómez, Livia; Pérez‐Jiménez, Jara; Casado, Marta; Clapés, Pere; Piña, Benjamín; Torres, Josep Lluís
• Is Part Of: Obesity. Volume 22:Number 4(2014:Apr.)
• Page Start: 976
• Page End: 979
• Publication Date: 2013-12-04
• Subjects: Obesity -- Periodicals; 616.398005
• DOI: 10.1002/oby.20640
• Languages: English
• ISSNs: 1930-7381

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="oby20640-sec-0001" sec-type="section"> <title>Objective</title> <p>Becoming overweight has been related to elevated levels of Enterobacteriales in the gut. <sc>d‐</sc>Fagomine is an iminosugar that has been shown to selectively agglutinate Enterobacteriales <italic>in vitro</italic>. The goal of this work is to establish whether <sc>d</sc>‐fagomine exerts a similar effect <italic>in vivo</italic> and whether this has any downstream consequences on weight gain.</p> </sec> <sec id="oby20640-sec-0002" sec-type="section"> <title>Methods</title> <p>The rats were fed a high‐fat high‐sucrose diet (HFHS) supplemented with <sc>d</sc>‐fagomine (or not; for comparison) or a standard diet for 5 weeks. The levels of total bacteria, Enterobacteriales and <italic>Escherichia coli</italic> were determined in fecal samples by performing quantitative real‐time polymerase chain reactions on DNA.</p> </sec> <sec id="oby20640-sec-0003" sec-type="section"> <title>Results</title> <p>Whereas the total levels of bacteria were independent of the diet, rats fed HFHS (without <sc>d</sc>‐fagomine) excreted significantly higher proportions of Enterobacteriales and <italic>E. coli</italic> than those fed a standard diet. The levels of Enterobacteriales and <italic>E. coli</italic> of the rats given HFHS with <sc>d</sc>‐fagomine were similar to those of the rats fed a standard diet. Compared to the standard group, rats fed HFHS with <sc>d</sc>‐fagomine gained significantly less weight (15.3%) than those fed HFHS (20.9%).</p> </sec> <sec id="oby20640-sec-0004" sec-type="section"> <title>Conclusion</title> <p> <sc>d</sc>‐Fagomine reduces the amount of Enterobacteriales excreted by rats fed HFHS and this may help to avert becoming obese.</p> </sec> </abstract>