Serum levels of arachidonic acid metabolites change during prostate cancer progression. Issue 6 (16th January 2014)

• Record Type: Journal Article
• Authors: Rodríguez‐Blanco, Giovanny; Burgers, Peter C.; Dekker, Lennard J. M.; IJzermans, Jan J. N.; Wildhagen, Mark F.; Schenk‐Braat, Ellen A. M.; Bangma, Chris H.; Jenster, Guido; Luider, Theo M.
• Is Part Of: Prostate. Volume 74:Issue 6(2014)
• Page Start: 618
• Page End: 627
• Publication Date: 2014-01-16
• Subjects: Prostate -- Diseases -- Periodicals; 616
• DOI: 10.1002/pros.22779
• Languages: English
• ISSNs: 0270-4137

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="pros22779-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p>Arachidonic acid (AA) pathway has been shown to play a role in the development and progression of prostate cancer (PCa). In this study we aimed to assess the changes in concentrations of hydroxyeicosatetraenoic acids (HETEs) in serum samples from patients diagnosed with PCa compared to controls.</p> </sec> <sec id="pros22779-sec-0002" sec-type="section"> <title>METHODS</title> <p>HETEs were determined using ultrahigh pressure liquid chromatography‐tandem mass spectrometry (UHPLC‐MS/MS).</p> </sec> <sec id="pros22779-sec-0003" sec-type="section"> <title>RESULTS</title> <p>Elevated concentrations of 5‐HETE, 8‐HETE, 11‐HETE and 15‐HETE were observed in 6 out of 20 patients diagnosed with PCa; no statistical differences with controls were observed for 12‐HETE and AA in the discovery set. An independent validation set composed of 222 samples divided in five groups ranging from subjects with low PSA and no PCa, to patients with advanced PCa was included. In 30% of the patients in the advanced PCa group, up to ten times higher concentrations of the same set of HETEs were observed with a significant concomitant decrease of the concentration of AA. Logistic regression and Kaplan–Meier curves illustrate that a decreased concentration of AA is a predictor of PCa biochemical recurrence after radical prostatectomy (RP).</p> </sec> <sec id="pros22779-sec-0004" sec-type="section"> <title>CONCLUSIONS</title> <p>From the present study we conclude that a significant association between AA and AA metabolites in serum and PCa progression exists, although serum concentrations of HETEs exhibited low sensitivity toward the diagnosis of PCa. <italic>Prostate</italic> 74:618–627, 2014. © 2014 Wiley Periodicals, Inc.</p> </sec> </abstract>