Signalling protein complexes isolated from primary human skin‐resident T cells can be analysed by Multiplex IP‐FCM. Issue 4 (April 2014)

• Record Type: Journal Article
• Authors: Smith, Stephen E. P.; Neier, Steven C.; Davis, Tessa R.; Pittelkow, Mark R.; Gil, Diana; Schrum, Adam G.
• Is Part Of: Experimental dermatology. Volume 23:Issue 4(2014:Apr.)
• Page Start: 272
• Page End: 273
• Publication Date: 2014-04
• Subjects: Dermatology -- Periodicals; 616.5
• DOI: 10.1111/exd.12362
• Languages: English
• ISSNs: 0906-6705

Abstract

<abstract abstract-type="main" id="exd12362-abs-0001"> <title>Abstract</title> <p>Studying signal transduction in skin‐resident T cells (sr‐T cells) can be limited by the small size of clinical biopsies. Here, we isolated sr‐T cells from clinical samples and analysed signalling protein complexes by multiplex immunoprecipitation detected by flow cytometry (mIP‐FCM). In samples from two independent donors, antigenic stimulation induced signalling proteins to join shared complexes that were observed in seven pairwise combinations among five proteins. This demonstrates that sr‐T cells isolated from small clinical samples provide sufficient material for mIP‐FCM‐based analysis of signalling‐induced protein complexes. We propose that this strategy may be useful for gaining improved mechanistic insight of sr‐T cell signal transduction associated with dermatological disease.</p> </abstract>