Ammonium improves elution of fixed dried blood spots without affecting immunofluorescence assay quality. Issue 4 (6th January 2014)

• Record Type: Journal Article
• Authors: Borremans, Benny
• Is Part Of: Tropical medicine & international health. Volume 19:Issue 4(2014:Apr.)
• Page Start: 413
• Page End: 416
• Publication Date: 2014-01-06
• Subjects: Tropical medicine -- Periodicals; Public health -- Periodicals; 616.988
• DOI: 10.1111/tmi.12259
• Languages: English
• ISSNs: 1360-2276

Abstract

<abstract abstract-type="main" id="tmi12259-abs-0001"> <title>Abstract</title> <sec id="tmi12259-sec-0001" sec-type="section"> <title>Objective</title> <p>To solve the problem of fixed dried blood spot elution without damaging IgG antibodies.</p> </sec> <sec id="tmi12259-sec-0002" sec-type="section"> <title>Methods</title> <p>The minimum effective concentration of liquid ammonium (NH₃) in a PBS solution, which was found to elute fixed blood, was determined. By using a dilution series, the effects of NH₃ on IgG antibody quality were assessed using immunofluorescence assays.</p> </sec> <sec id="tmi12259-sec-0003" sec-type="section"> <title>Results</title> <p>The minimum effective concentration of 0.2% NH₃ has no detectable effects on IgG quality.</p> </sec> <sec id="tmi12259-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Ammonium greatly improves blood elution from fixed DBS while maintaining IgG antibody quality. These results are encouraging and provide a basis for further testing of the efficacy of ammonium in different situations as well as its effect on other compounds.</p> </sec> </abstract>