Feline eosinophilic dermatoses: a retrospective immunohistochemical and ultrastructural study of extracellular matrix remodelling. Issue 2 (April 2014)

• Record Type: Journal Article
• Authors: Porcellato, Ilaria; Giontella, Andrea; Mechelli, Luca; Del Rossi, Emilia; Brachelente, Chiara
• Is Part Of: Veterinary dermatology. Volume 25:Issue 2(2014)
• Page Start: 86
• Page End: e26
• Publication Date: 2014-04
• Subjects: Veterinary dermatology -- Periodicals; Pet medicine -- Periodicals; 636.08965
• DOI: 10.1111/vde.12119
• Languages: English
• ISSNs: 0959-4493

Abstract

<abstract abstract-type="main" id="vde12119-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="vde12119-sec-0001" sec-type="section"> <title>Background</title> <p>Feline eosinophilic dermatoses (FEDs) are common diseases of cats with an unknown pathogenesis. They are histologically characterized by an eosinophilic infiltration and often by the presence of flame figures (FFs) and/or areas of loss of tissue architecture, here termed necrotic foci (NF). It has been postulated that an alteration in the degradation of the extracellular matrix could be responsible for these histological features. Matrix metalloproteinases (MMPs) are a group of proteases that are fundamental in extracellular matrix remodelling.</p> </sec> <sec id="vde12119-sec-0002" sec-type="section"> <title>Hypothesis/Objectives</title> <p>The aim of the study was to investigate retrospectively the expression of a subgroup of MMPs, in particular MMP‐2 and MMP‐9 gelatinases, in FEDs. The expression of one of their inhibitors, TIMP‐2, was also investigated in order to establish the role of these molecules in the pathogenesis of FEDs. The ultrastructural characteristics of extracellular matrix in FFs and NF were subsequently assessed.</p> </sec> <sec id="vde12119-sec-0003" sec-type="section"> <title>Methods</title> <p>Fifty‐one formalin‐fixed, paraffin‐embedded specimens from cutaneous and mucosal biopsies diagnosed as FEDs were investigated immunohistochemically. Two selected samples were processed for electron microscopy.</p> </sec> <sec id="vde12119-sec-0004" sec-type="section"> <title>Results</title> <p>This study revealed an increased expression of MMP‐2 in NF and a decreased expression of this gelatinase in FFs. An imbalance between MMP‐2 and TIMP‐2 was evident using immunohistochemistry. No significative results were observed for MMP‐9 expression. Electron microscopy confirmed the lack of normal collagen fibres in NF, whereas in FFs only occasional, amorphous material was observed among normal collagen fibres.</p> </sec> <sec id="vde12119-sec-0005" sec-type="section"> <title>Conclusions and clinical importance</title> <p>Our study suggests that an imbalance in the expression of matrix metalloproteinases could be responsible for different morphological findings in FEDs. Further studies are needed to assess the role of matrix metalloproteinases in the pathogenesis of FEDs.</p> </sec> </abstract>