Gastric secretion does not affect the reliability of the 13C‐acetate breath test: A validation of the 13C‐acetate breath test by magnetic resonance imaging. Issue 2 (16th October 2012)

• Record Type: Journal Article
• Authors: Kuyumcu, S.; Goetze, O.; Menne, D.; Treier, R.; Boesiger, P.; Fox, M.; Fried, M.; Schwizer, W.; Steingoetter, A.
• Is Part Of: Neurogastroenterology & motility. Volume 25:Issue 2(2013:Feb.)
• Page Start: 176
• Page End: e87
• Publication Date: 2012-10-16
• Subjects: Gastrointestinal system -- Motility -- Periodicals; Gastrointestinal system -- Innervation -- Periodicals; 616.33
• DOI: 10.1111/nmo.12025
• Languages: English
• ISSNs: 1350-1925

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p> <bold>Background </bold> <sup>13</sup>C‐Acetate labeled meals are widely used to determine meal emptying by means of analyzing resulting <sup>13</sup>CO<sub>2</sub> exhalation dynamics. In contrast to the underlying metabolic processes, only few <sup>13</sup>C breath test meal emptying studies have focused on intragastric processes that may alter <sup>13</sup>CO<sub>2</sub> exhalation. This work assessed the effect of enhanced gastric secretion on the reliability of half emptying time (<italic>t</italic>50) measurements by <sup>13</sup>C‐acetate breath test.</p> <p> <bold>Methods </bold> <sup>13</sup>CO<sub>2</sub> exhalation data were acquired in a double‐blind, randomized, cross‐over gastric emptying study in 12 healthy volunteers receiving either pentagastrin or placebo intravenously. The standard method proposed by Ghoos <italic>et al.</italic> was applied to calculate <italic>t</italic>50 (<italic>t</italic>50_Ghoos) from <sup>13</sup>CO<sub>2</sub> exhalation data, which were compared and tested for agreement to meal half emptying times (<italic>t</italic>50_MV) from concurrent recorded MRI (magnetic resonance imaging) volume data. In addition, the accumulated gastric secretion volumes during infusion as detected by MRI (AUC_SV<sub>60</sub>) were correlated with the corresponding cumulative percent <sup>13</sup>C doses recovered (cPDR<sub>60</sub>).</p> <p> <bold>Key Results </bold> <italic>t</italic>50_Ghoos and <italic>t</italic>50_MV showed a linear correlation with a slope of 1.1 ± 0.3 (<italic>r</italic><sup>2</sup> = 0.67), however, a positive offset of 136 min for <italic>t</italic>50_Ghoos. No correlation was detected between AUC_SV<sub>60</sub> and cPDR<sub>60</sub> (<italic>r</italic><sup>2</sup> = 0.11). Both, breath test and MRI, revealed a prolonged <italic>t</italic>50 under pentagastrin infusion with median differences in <italic>t</italic>50_Ghoos of 45[28–84] min (<italic>P</italic> = 0.002) and <italic>t</italic>50_MV of 39[28–52] min (<italic>P</italic> = 0.002).</p> <p> <bold>Conclusions & Inferences </bold> This study suggests that <sup>13</sup>CO<sub>2</sub> exhalation after ingestion of a <sup>13</sup>C‐labeled liquid test meal is not affected by stimulated gastric secretion, but is rather reflecting the dynamics of meal or caloric emptying from the stomach.</p> </abstract>