Marker assisted selection of low phytic acid trait in maize (Zea mays L.). Issue 1 (13th March 2014)

• Record Type: Journal Article
• Authors: Sureshkumar, S.; Tamilkumar, P.; Senthil, N.; Nagarajan, P.; Thangavelu, A. U.; Raveendran, M.; Vellaikumar, S.; Ganesan, K. N.; Balagopal, R.; Vijayalakshmi, G.; Shobana, V.
• Is Part Of: Hereditas. Volume 151:Issue 1(2014:Feb.)
• Page Start: 20
• Page End: 27
• Publication Date: 2014-03-13
• Subjects: Heredity -- Periodicals; Genetics -- Periodicals; Cytogénétique; Génétique; Hérédité; Périodique électronique (Descripteur de forme); Ressource Internet (Descripteur de forme); 576.5
• DOI: 10.1111/j.1601-5223.2013.00030.x
• Languages: English
• ISSNs: 0018-0661

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Maize is the third important major food crop. Breeding for low phytate maize genotypes is an effective strategy for decreasing the content of kernel phytic acid (a chelator of cations such as Ca<sup>2+</sup> and Fe<sup>3+</sup>) and thereby increasing the bioavailability of nutritive minerals in human diet and animal feed. Previous studies have established that a mutant plant with a <italic>lpa2‐2</italic> allele accumulates less phytic acid in seeds. Therefore, the marker assisted backcross breeding (MABB), which involves introgression of <italic>lpa2‐2</italic> recessive allele (which confer low phytate trait) from a <italic>lpa2‐2</italic> mutant line into a well‐adapted line using backcrosses and selection of lines possessing <italic>lpa2‐2</italic> allele in each backcross population using molecular markers, is an effective strategy for developing low phytate maize. So far, no studies have developed any <italic>lpa2‐2</italic> allele specific molecular markers for this purpose. Here, using backcross and selfed progenies, obtained by crossing low phytate mutant line 'EC 659418' (i.e. donor of <italic>lpa2‐2</italic> allele) into agronomically superior line 'UMI395', we have validated that a SSR marker 'umc2230', located 0.4 cM downstream of <italic>lpa2‐2</italic>, cosegregate, in a Mendelian fashion, with low phytic acid trait. Therefore umc2230 can be dependably used in MABB for the development of low phytate maize.</p> </abstract>