Polycationic Pillar[5]arene Derivatives: Interaction with DNA and Biological Applications. Issue 51 (11th November 2013)

• Record Type: Journal Article
• Authors: Nierengarten, Iwona; Nothisen, Marc; Sigwalt, David; Biellmann, Thomas; Holler, Michel; Remy, Jean‐Serge; Nierengarten, Jean‐François
• Is Part Of: Chemistry. Volume 19:Issue 51(2013)
• Page Start: 17552
• Page End: 17558
• Publication Date: 2013-11-11
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201303029
• Languages: English
• ISSNs: 0947-6539

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Dendritic pillar[5]arene derivatives have been efficiently prepared by grafting dendrons with peripheral Boc‐protected amine subunits onto a preconstructed pillar[5]arene scaffold. Upon cleavage of the Boc‐protected groups, water‐soluble pillar[5]arene derivatives with 20 (<bold>13</bold>) and 40 (<bold>14</bold>) peripheral ammonium groups have been obtained. The capability of these compounds to form stable nanoparticles with plasmid DNA has been demonstrated by gel electrophoresis, transmission electron microscopy (TEM), and dynamic light scattering (DLS) investigations. Transfection efficiencies of the self‐assembled <bold>13</bold>/pCMV‐Luc and <bold>14</bold>/pCMV‐Luc polyplexes have been evaluated in vitro with HeLa cells. The transfection efficiencies found for both compounds are good, and pillar[5]arenes <bold>13</bold> and <bold>14</bold> show very low toxicity if any.</p> </abstract>