Biosynthesis of Hygrocins, Antitumor Naphthoquinone Ansamycins Produced by Streptomyces sp. LZ35. Issue 1 (25th November 2013)

• Record Type: Journal Article
• Authors: Li, Shanren; Wang, Haoxin; Li, Yaoyao; Deng, Jingjing; Lu, Chunhua; Shen, Yan; Shen, Yuemao
• Is Part Of: Chembiochem. Volume 15:Issue 1(2014)
• Page Start: 94
• Page End: 102
• Publication Date: 2013-11-25
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201300599
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Hygrocins are naphthoquinone ansamycins with significant antitumor activities. Here, we report the identification and characterization of the hygrocin biosynthetic gene cluster (hgc) in <italic>Streptomyces</italic> sp. LZ35. A biosynthetic pathway is proposed based on bioinformatics analysis of the <italic>hgc</italic> genes and intermediates accumulated in selected gene disruption mutants. One of the steps during the biosynthesis of hygrocins is a Baeyer–Villiger oxidation between C5 and C6, catalyzed by luciferase‐like monooxygenase homologue Hgc3. Hgc3 represents the founding member of a previously uncharacterized family of enzymes acting as Baeyer–Villiger monooxygenases.</p> </abstract>