Identification and Biosynthesis of New Acyloins from the Thermophilic Bacterium Thermosporothrix hazakensis SK20‐1T. Issue 4 (28th January 2014)

• Record Type: Journal Article
• Authors: Park, Jin‐Soo; Kagaya, Noritaka; Hashimoto, Junko; Izumikawa, Miho; Yabe, Shuhei; Shin‐ya, Kazuo; Nishiyama, Makoto; Kuzuyama, Tomohisa
• Is Part Of: Chembiochem. Volume 15:Issue 4(2014)
• Page Start: 527
• Page End: 532
• Publication Date: 2014-01-28
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201300690
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Two new acyloin compounds were isolated from the thermophilic bacterium <italic>Thermosporothrix hazakensis</italic> SK20‐1^T. Genome sequencing of the bacterium and biochemical studies identified the thiamine diphosphate (TPP)‐dependent enzyme Thzk0150, which is involved in the formation of acyloin. Through extensive analysis of the Thzk0150‐catalyzed reaction products, we propose a putative reaction mechanism involving two substrates: 4‐methyl‐2‐oxovalerate as an acyl donor and phenyl pyruvate as an acyl acceptor.</p> </abstract>