Novel Aeruginosin‐865 from Nostoc sp. as a Potent Anti‐inflammatory Agent. Issue 17 (2nd October 2013)

• Record Type: Journal Article
• Authors: Kapuścik, Aleksandra; Hrouzek, Pavel; Kuzma, Marek; Bártová, Simona; Novák, Petr; Jokela, Jouni; Pflüger, Maren; Eger, Andreas; Hundsberger, Harald; Kopecký, Jiří
• Is Part Of: Chembiochem. Volume 14:Issue 17(2013)
• Page Start: 2329
• Page End: 2337
• Publication Date: 2013-10-02
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201300246
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Aeruginosin‐865 (Aer‐865), isolated from terrestrial cyanobacterium <italic>Nostoc</italic> sp. Lukešová 30/93, is the first aeruginosin‐type peptide containing both a fatty acid and a carbohydrate moiety, and is the first aeruginosin to be found in the genus <italic>Nostoc</italic>. Mass spectrometry, chemical and spectroscopic analysis as well as one‐ and two‐dimensional NMR and chiral HPLC analysis of Marfey derivatives were applied to determine the peptidic sequence: <sc>D</sc>‐Hpla, <sc>D</sc>‐Leu, 5‐OH‐Choi, Agma, with hexanoic and mannopyranosyl uronic acid moieties linked to Choi. We used an AlphaLISA assay to measure the levels of proinflammatory mediators IL‐8 and ICAM‐1 in hTNF‐α‐stimulated HLMVECs. Aer‐865 showed significant reduction of both: with EC₅₀ values of (3.5±1.5) μg mL⁻¹ ((4.0±1.7) μ<sc>M</sc>) and (50.0±13.4) μg mL⁻¹ ((57.8±15.5) μ<sc>M</sc>), respectively. Confocal laser scanning microscopy revealed that the anti‐inflammatory effect of Aer‐865 was directly associated with inhibition of NF‐κB translocation to the nucleus. Moreover, Aer‐865 did not show any cytotoxic effect.</p> </abstract>