RevErbα Preferentially Deforms DNA by Induced Fit. Issue 5 (23rd February 2014)

• Record Type: Journal Article
• Authors: Chung, Ying‐Hua; van der Vaart, Arjan
• Is Part Of: Chembiochem. Volume 15:Issue 5(2014)
• Page Start: 643
• Page End: 646
• Publication Date: 2014-02-23
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201300557
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Using free‐energy simulations, we have shown that RevErbα‐induced DNA deformation preferentially occurs by induced fit rather than by conformational selection, even though the DNA is only slightly distorted in the complex. Our study shows that information on the sequence of binding events is needed to establish whether conformational selection or induced fit is operative, and that the presence of multiple apo‐state structures might not be enough to distinguish between these binding models.</p> </abstract>