An Unconventional Glycosyl Transfer Reaction: Glucansucrase GTFA Functions as an Allosyltransferase Enzyme. Issue 18 (13th November 2013)

• Record Type: Journal Article
• Authors: Timm, Malte; Görl, Julian; Kraus, Michael; Kralj, Slavko; Hellmuth, Hendrik; Beine, Raphael; Buchholz, Klaus; Dijkhuizen, Lubbert; Seibel, Jürgen
• Is Part Of: Chembiochem. Volume 14:Issue 18(2013)
• Page Start: 2423
• Page End: 2426
• Publication Date: 2013-11-13
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201300392
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>First reported allosyltransferase:</bold> The allopyranosyl analogue of sucrose (All‐Fru) was used as donor for the glucansucrase GTFA from <italic>Lactobacillus reuteri</italic> 121. This enzyme is able to hydrolyse All‐Fru and transfer allose to acceptor substrates such as sugars, amino acids and (−)‐epicatechin, thus forming artificial α‐linked allopyranosyl products. A triple GTFA mutant showed twofold increase in allosyl transfer.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg4t3m3drk" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>