Chemoselective Immobilization of Proteins by Microcontact Printing and Bio‐orthogonal Click Reactions. Issue 18 (25th October 2013)

• Record Type: Journal Article
• Authors: Tolstyka, Zachary P.; Richardson, Wade; Bat, Erhan; Stevens, Caitlin J.; Parra, Dayanara P.; Dozier, Jonathan K.; Distefano, Mark D.; Dunn, Bruce; Maynard, Heather D.
• Is Part Of: Chembiochem. Volume 14:Issue 18(2013)
• Page Start: 2464
• Page End: 2471
• Publication Date: 2013-10-25
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201300478
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Herein, a combination of microcontact printing of functionalized alkanethiols and site‐specific modification of proteins is utilized to chemoselectively immobilize proteins onto gold surfaces, either by oxime‐ or copper‐catalyzed alkyne–azide click chemistry. Two molecules capable of click reactions were synthesized, an aminooxy‐functionalized alkanethiol and an azide‐functionalized alkanethiol, and self‐assembled monolayer (SAM) formation on gold was confirmed by IR spectroscopy. The alkanethiols were then individually patterned onto gold surfaces by microcontact printing. Site‐specifically modified proteins—horse heart myoglobin (HHMb) containing an N‐terminal α‐oxoamide and a red fluorescent protein (mCherry‐CVIA) with a C‐terminal alkyne—were immobilized by incubation onto respective stamped functionalized alkanethiol patterns. Pattern formation was confirmed by fluorescence microscopy.</p> </abstract>