Adaptation of Dekkera bruxellensis to lignocellulose‐based substrate. (January 2014)

• Record Type: Journal Article
• Authors: Tiukova, Ievgeniia A.; de, Will; Sundell, David; Haddad Momeni, Majid; Horn, Svein Jarle; Ståhlberg, Jerry; de, Marcos Antonio; Passoth, Volkmar; Rapoport, Alexander; Heipieper, Hermann J.
• Is Part Of: Biotechnology and applied biochemistry. Volume 61:Number 1(2014:Jan./Feb.)
• Page Start: 51
• Page End: 57
• Publication Date: 2014-01
• Subjects: Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.1145
• Languages: English
• ISSNs: 0885-4513

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Adaptation of <italic>Dekkera bruxellensis</italic> to lignocellulose hydrolysate was investigated. Cells of <italic>D. bruxellensis</italic> were grown for 72 and 192 H in batch and continuous culture, respectively (adapted cells). Cultivations in semisynthetic medium were run as controls (nonadapted cells). To test the adaptation, cells from these cultures were reinoculated in the lignocellulose medium, and growth and ethanol production characteristics were monitored. Cells adapted to lignocellulose hydrolysate had a shorter lag phase, grew faster, and produced a higher ethanol concentration as compared with nonadapted cells. A stability test showed that after cultivation in rich medium, cells partially lost the adapted phenotype but still showed faster growth and higher ethanol production as compared with nonadapted cells. Because alcohol dehydrogenase genes have been described to be involved in the adaptation to furfural in <italic>Saccharomyces cerevisiae</italic>, an analogous mechanism of adaptation to lignocelluloses hydrolysate of <italic>D. bruxellensis</italic> was hypothesized. However, gene expression analysis showed that genes homologous to <italic>S. cerevisiae ADH1</italic> were not involved in the adaptation to lignocelluloses hydrolysate in <italic>D. bruxellensis</italic>.</p> </abstract>