Ex vivo cytokine production in peripheral blood mononuclear cells after their stimulation with dsRNA of natural origin. (30th January 2014)

• Record Type: Journal Article
• Authors: Veinalde, Rūta; Petrovska, Ramona; Brūvere, Rūta; Feldmane, Guna; Pjanova, Dace; Rapoport, Alexander; Heipieper, Hermann J.
• Is Part Of: Biotechnology and applied biochemistry. Volume 61:Number 1(2014:Jan./Feb.)
• Page Start: 65
• Page End: 73
• Publication Date: 2014-01-30
• Subjects: Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.1143
• Languages: English
• ISSNs: 0885-4513

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Double‐stranded RNA (dsRNA) is a pathogen‐associated molecular pattern, known for its ability to induce antiviral response and enhance communication between cells mediating innate and adaptive immune responses. The aim of this study was to characterize the effect of the dsRNA‐containing product Larifan on the production of a wide spectrum of cytokines and chemokines in <italic>ex vivo</italic> cultivated peripheral blood mononuclear cells. Concentrations of 29 different cytokines were detected by a Luminex® 200™ System using three Milliplex MAP Multiplex Assay Kits. Larifan caused strong induction of chemokine macrophage inflammatory protein 1<alternatives><inline-graphic mimetype="image" xlink:href="ark:/27927/pgg4srmkxrk" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /><mml:math display="inline" altimg="urn:x-wiley:08854513:bab1143:equation:bab1143-math-0001" overflow="scroll" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>β</mml:mi></mml:math></alternatives>, I‐309, and TARC, proinflammatory cytokines IL‐6, tumor necrosis factor ‐<alternatives><inline-graphic mimetype="image" xlink:href="ark:/27927/pgg4srmkxq1" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /><mml:math display="inline" altimg="urn:x-wiley:08854513:bab1143:equation:bab1143-math-0002" overflow="scroll" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>α</mml:mi></mml:math></alternatives>, granulocyte macrophage colony‐stimulating factor, anti‐inflammatory IL‐10, and cellular immunity mediating factors IL‐23 and interferon‐<alternatives><inline-graphic mimetype="image" xlink:href="ark:/27927/pgg4srmkxpg" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /><mml:math display="inline" altimg="urn:x-wiley:08854513:bab1143:equation:bab1143-math-0003" overflow="scroll" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>γ</mml:mi></mml:math></alternatives>. Considerable suppression of IL‐16 and chemokine stromal cell‐derived factor 1 a+b and interferon gamma‐induced protein 10 was also observed. The network of molecules responding to the presence of Larifan revealed the pleiotropic effect this product exerts on immune response.</p> </abstract>