Ultrasensitive and Fast Bottom‐up Analysis of Femtogram Amounts of Complex Proteome Digests1. Issue 51 (31st October 2013)

• Record Type: Journal Article
• Authors: Sun, Liangliang; Zhu, Guijie; Zhao, Yimeng; Yan, Xiaojing; Mou, Si; Dovichi, Norman J.
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 51(2013)
• Page Start: 13661
• Page End: 13664
• Publication Date: 2013-10-31
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201308139
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Femtogram proteomics</bold>: An ultrasensitive capillary zone electrophoresis–mass spectrometry system that is based on an improved nanospray interface has been developed. This system is used for the analysis of picogram to femtogram amounts of <italic>E. coli</italic> digests; for example, over 100 proteins were identified from 16 pg digests by tandem mass spectrometry. AMTs=accurate mass and time tags.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg4t3jf7dd" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>