High‐Fidelity Protein Targeting into Membrane Lipid Microdomains in Living Cells1. Issue 5 (18th December 2013)

• Record Type: Journal Article
• Authors: Beutel, Oliver; Nikolaus, Jörg; Birkholz, Oliver; You, Changjiang; Schmidt, Thomas; Herrmann, Andreas; Piehler, Jacob
• Is Part Of: Angewandte Chemie international edition. Volume 53:Issue 5(2014)
• Page Start: 1311
• Page End: 1315
• Publication Date: 2013-12-18
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201306328
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Lipid analogues carrying three nitrilotriacetic acid (tris‐NTA) head groups were developed for the selective targeting of His‐tagged proteins into liquid ordered (l_o) or liquid disordered (l_d) lipid phases. Strong partitioning into the l_o phase of His‐tagged proteins bound to tris‐NTA conjugated to saturated alkyl chains (tris‐NTA DODA) was achieved, while tris‐NTA conjugated to an unsaturated alkyl chain (tris‐NTA SOA) predominantly resided in the l_d phase. Interestingly, His‐tag‐mediated lipid crosslinking turned out to be required for efficient targeting into the l_o phase by tris‐NTA DODA. Robust partitioning into l_o phases was confirmed by using viral lipid mixtures and giant plasma membrane vesicles. Moreover, efficient protein targeting into l_o and l_d domains within the plasma membrane of living cells was demonstrated by single‐molecule tracking, thus establishing a highly generic approach for exploring lipid microdomains in situ.</p> </abstract>