Regulation of the Cyanobacterial Circadian Clock by Electrochemically Controlled Extracellular Electron Transfer1. (4th February 2014)

• Record Type: Journal Article
• Authors: Lu, Yue; Nishio, Koichi; Matsuda, Shoichi; Toshima, Yuki; Ito, Hiroshi; Konno, Tomohiro; Ishihara, Kazuhiko; Kato, Souichiro; Hashimoto, Kazuhito; Nakanishi, Shuji
• Is Part Of: Angewandte Chemie. Volume 126:Number 8(2014)
• Page Start: 2240
• Page End: 2243
• Publication Date: 2014-02-04
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201309560
• Languages: English
• ISSNs: 0044-8249

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>There is growing awareness that circadian clocks are closely related to the intracellular redox state across a range of species. As the redox state is determined by the exchange of the redox species, electrochemically controlled extracellular electron transfer (EC‐EET), a process in which intracellular electrons are exchanged with extracellular electrodes, is a promising approach for the external regulation of circadian clocks. Herein, we discuss whether the circadian clock can be regulated by EC‐EET using the cyanobacterium <italic>Synechococcus elongatus</italic> PCC7942 as a model system. In vivo monitoring of chlorophyll fluorescence revealed that the redox state of the plastoquionone pool could be controlled with EC‐EET by simply changing the electrode potential. As a result, the endogenous circadian clock of <italic>S. elongatus</italic> cells was successfully entrained through periodically modulated EC‐EET by emulating the natural light/dark cycle, even under constant illumination conditions. This is the first example of regulating the biological clock by electrochemistry.</p> </abstract>