Noradrenaline and neuropeptide Y contribute to initial, but not sustained, vasodilatation in response to local skin warming in humans. Issue 2 (16th December 2013)

• Record Type: Journal Article
• Authors: Hodges, Gary J.; Sparks, Paul A.
• Is Part Of: Experimental physiology. Volume 99:Issue 2(2014:Feb.)
• Page Start: 381
• Page End: 392
• Publication Date: 2013-12-16
• Subjects: Physiology, Experimental -- Periodicals; 571.0724
• DOI: 10.1113/expphysiol.2013.075549
• Languages: English
• ISSNs: 0958-0670

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="eph1388-sec-0010" sec-type="section"> <title>New Findings</title> <p> <list id="eph1388-list-0001" list-type="bullet"> <list-item> <p> <bold>What is the central question of this study?</bold> </p> <p>Previous work has produced the counterintuitive finding that the vasoconstrictor neurotransmitters noradrenaline and neuropeptide Y are involved in vasodilatation. We aimed to discover whether sympathetic neurotransmitters are required for the sustained vasodilatation in response to local skin warming, as has been previously suggested, and to determine whether noradrenaline and neuropeptide Y are 'mediating' the sustained vasodilator response directly or acting to 'prime' (or kick‐start) it.</p> </list-item> <list-item> <p> <bold>What is the main finding and its importance?</bold> </p> <p>We have found that noradrenaline and neuropeptide Y are required at the initiation of vasodilatation in response to local skin warming, if a complete vasodilator response is to be achieved; however, they are not required once vasodilatation has begun.</p> </list-item> </list> </p> </sec> <sec id="eph1388-sec-0020" sec-type="section"> <p>In a three‐part study, we examined whether noradrenaline, neuropeptide Y (NPY) and endothelial nitric oxide synthase (eNOS) were involved in the sustained vasodilatation in response to local skin warming. Forearm skin sites were instrumented with intradermal microdialysis fibres, local skin heaters and laser‐Doppler flow probes. Local skin temperature (<italic>T</italic><sub>loc</sub>) was increased from 34 to 42°C at a rate of 0.5°C (10 s)<sup>−1</sup>. Laser‐Doppler flow was expressed as cutaneous vascular conductance (CVC; laser‐Doppler flow/mean arterial pressure). In part 1, three skin sites were prepared; two were treated with the study vehicle (lactated Ringer solution), while the third site was treated with yohimbine and propranolol to antagonize <italic>α</italic>‐ and <italic>β</italic>‐receptors, and 10 min of baseline data were record at a <italic>T</italic><sub>loc</sub> of 34°C. Receptor antagonism was confirmed via infusion of clonidine. The <italic>T</italic><sub>loc</sub> was increased to 42°C at all sites. Once CVC had stabilized, site 2 was treated with yohimbine and propranolol to examine the effect of adrenergic receptor blockade on sustained vasodilatation of the skin. Receptor antagonism was again confirmed via infusion of clonidine. All sites were treated with sodium nitroprusside, and <italic>T</italic><sub>loc</sub> was increased to 43°C to elicit maximal vasodilatation. In parts 2 and 3, the general protocol was the same, except that BIBP‐3226 was used to antagonize Y<sub>1</sub>‐receptors, NPY to test the efficacy of the antagonism, <italic>N</italic><sup>G</sup>‐amino‐<sc>l</sc>‐arginine to inhibit eNOS and ACh to test the adequacy of inhibition. Compared with control conditions, antagonism of <italic>α</italic>‐ and <italic>β</italic>‐receptors, Y<sub>1</sub>‐receptors and eNOS before local skin warming reduced the initial and sustained vasodilatation in response to increased <italic>T</italic><sub>loc</sub>. However, treatment with yohimbine and propranolol or BIBP‐3226 after local skin warming did not affect the sustained vasodilatation [CVC, 90 ± 3 <italic>versus</italic> 89 ± 3%max (control <italic>vs</italic>. yohimbine and propranolol) and 88 ± 5 <italic>versus</italic> 87 ± 4%max (control <italic>vs</italic>. BIBP‐3226); <italic>P</italic> > 0.05]. <italic>N</italic><sup>G</sup>‐Amino‐<sc>l</sc>‐arginine perfusion caused a large reduction in CVC during this phase (89 ± 5 <italic>versus</italic> 35 ± 4%max; <italic>P</italic> < 0.05). These data indicate that if their actions are antagonized after local warming and cutaneous vasodilatation has occurred, noradrenaline and NPY play little, if any, role in the sustained vasodilatation in response to local skin warming. However, eNOS contributes markedly to the sustained vasodilatation regardless of when it is inhibited.</p> </sec> </abstract>