The symbiotic transcription factor MtEFD and cytokinins are positively acting in the Medicago truncatula and Ralstonia solanacearum pathogenic interaction. Issue 4 (11th December 2013)

• Record Type: Journal Article
• Authors: Moreau, Sandra; Fromentin, Justine; Vailleau, Fabienne; Vernié, Tatiana; Huguet, Stéphanie; Balzergue, Sandrine; Frugier, Florian; Gamas, Pascal; Jardinaud, Marie‐Françoise
• Is Part Of: New phytologist. Volume 201:Issue 4(2014)
• Page Start: 1343
• Page End: 1357
• Publication Date: 2013-12-11
• Subjects: Botany -- Periodicals; 580
• DOI: 10.1111/nph.12636
• Languages: English
• ISSNs: 0028-646X

Abstract

<abstract abstract-type="main" id="nph12636-abs-0001"> <title>Summary</title> <p> <list id="nph12636-list-0001" list-type="bullet"> <list-item> <p>A plant–microbe dual biological system was set up involving the model legume <italic>Medicago truncatula</italic> and two bacteria, the soil‐borne root pathogen <italic>Ralstonia solanacearum</italic> and the beneficial symbiont <italic>Sinorhizobium meliloti</italic>.</p> </list-item> <list-item> <p>Comparison of transcriptomes under symbiotic and pathogenic conditions highlighted the transcription factor Mt<italic>EFD</italic> (Ethylene response Factor required for nodule Differentiation) as being upregulated in both interactions, together with a set of cytokinin‐related transcripts involved in metabolism, signaling and response. Mt<italic>RR4</italic> (Response Regulator), a cytokinin primary response gene negatively regulating cytokinin signaling and known as a target of Mt<italic>EFD</italic> in nodulation processes, was retrieved in this set of transcripts.</p> </list-item> <list-item> <p>Refined studies of Mt<italic>EFD</italic> and Mt<italic>RR4</italic> expression during <italic>M. truncatula</italic> and <italic>R. solanacearum</italic> interaction indicated differential kinetics of induction and requirement of central regulators of bacterial pathogenicity, HrpG and HrpB. Similar to Mt<italic>RR4</italic>, Mt<italic>EFD</italic> upregulation during the pathogenic interaction was dependent on cytokinin perception mediated by the Mt<italic>CRE1</italic> (Cytokinin REsponse 1) receptor.</p> </list-item> <list-item> <p>The use of <italic>M. truncatula efd‐1</italic> and <italic>cre1‐1</italic> mutants evidenced Mt<italic>EFD</italic> and cytokinin perception as positive factors for bacterial wilt development. These factors therefore play an important role in both root nodulation and root disease development.</p> </list-item> </list> </p> </abstract>