The bacterial effector HopM1 suppresses PAMP‐triggered oxidative burst and stomatal immunity. Issue 1 (23rd December 2013)

• Record Type: Journal Article
• Authors: Lozano‐Durán, Rosa; Bourdais, Gildas; He, Sheng Yang; Robatzek, Silke
• Is Part Of: New phytologist. Volume 202:Issue 1(2014)
• Page Start: 259
• Page End: 269
• Publication Date: 2013-12-23
• Subjects: Botany -- Periodicals; 580
• DOI: 10.1111/nph.12651
• Languages: English
• ISSNs: 0028-646X

Abstract

<abstract abstract-type="main" id="nph12651-abs-0001"> <title>Summary</title> <p> <list id="nph12651-list-0001" list-type="bullet"> <list-item> <p>Successful pathogens counter immunity at multiple levels, mostly through the action of effectors. <italic>Pseudomonas syringae</italic> secretes <italic>c</italic>. 30 effectors, some of which have been shown to inhibit plant immunity triggered upon perception of conserved pathogen‐associated molecular patterns (PAMPs). One of these is HopM1, which impairs late immune responses through targeting the vesicle trafficking‐related AtMIN7 for degradation.</p> </list-item> <list-item> <p>Here, we report that <italic>in planta</italic> expressed HopM1 suppresses two early PAMP‐triggered responses, the oxidative burst and stomatal immunity, both of which seem to require proteasomal function but are independent of AtMIN7. Notably, a 14‐3‐3 protein, GRF8/AtMIN10, was found previously to be a target of HopM1 <italic>in vivo</italic>, and expression of HopM1 mimics the effect of chemically and genetically disrupting 14‐3‐3 function.</p> </list-item> <list-item> <p>Our data further show that the function of 14‐3‐3 proteins is required for PAMP‐triggered oxidative burst and stomatal immunity, and chemical‐mediated disruption of the 14‐3‐3 interactions with their client proteins restores virulence of a HopM1‐deficient <italic>P. syringae</italic> mutant, providing a link between HopM1 and the involvement of 14‐3‐3 proteins in plant immunity.</p> </list-item> <list-item> <p>Taken together, these results unveil the impact of HopM1 on the PAMP‐triggered oxidative burst and stomatal immunity in an AtMIN7‐independent manner, most likely acting at the function of (a) 14‐3‐3 protein(s).</p> </list-item> </list> </p> </abstract>